Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Silver stained 2D-gels of protein extracts from wild type and Hsp70-mutant yeast strains
type: Dataset
distribution: Landing page for the dataset
title: Studier av det cytosoliska Hsp70-systemet från jäst och dess roll i proteinhomeostas och reglering av livslängd - Silverfärgade 2D-geler av proteinextrakt från vildtyp och Hsp70-mutanta jäststammar @sv
description: Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts. Images of two dimensional protein gels with protein extracts from wild type and Hsp70 mutant yeast strains in mid-exponential growth phase. The yeast were grown in rich medium (YP) with 2 % glucose as carbon source. The images have been aligned and the background has been removed. The dataset was collected through imaging of two dimensional protein gels. The image files are provided in TIFF format.
description: Vi har systematiskt studerat vilka funktioner hos de cytosoliska molekylära chaperonerna av klassen Hsp70 hos jäst som krävs för att upprätthålla en normal livslängd. Bilder av tvådimensionella proteingeler med proteinextrakt från vildtyp och Hsp70-mutanta jäststammar i mitten av den exponentiella tillväxtfasen. Jästen har odlats i rikt medium (YP) med 2 % glukos som kolkälla. Datasetet insamlades via fotografering av tvådimensionella proteingeler. Bildfilerna är i TIFF-format. @sv
modified: 2021-02-11T14:13:18.095445Z
issued: 2020-12-16T11:37:40.689617Z
publisher: University of Gothenburg
theme: HEAL
access rights: PUBLIC
qualified attribution: Attribution
  had role: principalInvestigator
  agent: David Öling
    type: Agent
    account: https://orcid.org/0000-0002-3390-3543
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Rebecca Andersson
    type: Agent
    account: https://orcid.org/0000-0003-3765-5038
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Thomas Nyström
    type: Agent
    account: https://orcid.org/0000-0001-5489-2903
    type: PrivateIndividual(s)
contact point: Individual
  fn: Thomas Nyström
keyword: Cellular Senescence
keyword: Cellåldrande @sv
keyword: Molecular Chaperones
keyword: Molekylära chaperoner @sv
keyword: HSP70 Heat-Shock Proteins
keyword: HSP70-värmechockproteiner @sv
keyword: Proteostasis Deficiencies
keyword: Proteostasbrist @sv
temporal: PeriodOfTime
  start date: 2012
  end date: 2020
language: ENG

Landing page for the dataset
type: Distribution
title: Landningssida för datasetet @sv
access URL: Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Silver stained 2D-gels of protein extracts from wild type and Hsp70-mutant yeast strains
availability: stable

University of Gothenburg
type: Agent
name: Göteborgs universitet @sv
type: Academia-ScientificOrganisation
source: 01tm6cn81