Studier av det cytosoliska Hsp70-systemet från jäst och dess roll i proteinhomeostas och reglering av livslängd - Silverfärgade 2D-geler av proteinextrakt från vildtyp och Hsp70-mutanta jäststammar Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Silver stained 2D-gels of protein extracts from wild type and Hsp70-mutant yeast strains 2020-36-11-1 https://doi.org/10.5878/9xxe-g981 Swedish National Data Service Svensk nationell datatjänst Landing page Studier av det cytosoliska Hsp70-systemet från jäst och dess roll i proteinhomeostas och reglering av livslängd - Silverfärgade 2D-geler av proteinextrakt från vildtyp och Hsp70-mutanta jäststammar Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Silver stained 2D-gels of protein extracts from wild type and Hsp70-mutant yeast strains 2020-36-11-1 https://doi.org/10.5878/9xxe-g981 10.1101/2020.06.25.170670 10.1371/journal.pgen.1008951 Öling, David Öling, David Andersson, Rebecca Andersson, Rebecca Nyström, Thomas Nyström, Thomas Swedish National Data Service Svensk nationell datatjänst Landing page Cellular Senescence Cellåldrande Molecular Chaperones Molekylära chaperoner HSP70 Heat-Shock Proteins HSP70-värmechockproteiner Proteostasis Deficiencies Proteostasbrist Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts. Images of two dimensional protein gels with protein extracts from wild type and Hsp70 mutant yeast strains in mid-exponential growth phase. The yeast were grown in rich medium (YP) with 2 % glucose as carbon source. The images have been aligned and the background has been removed. The dataset was collected through imaging of two dimensional protein gels. The image files are provided in TIFF format. Vi har systematiskt studerat vilka funktioner hos de cytosoliska molekylära chaperonerna av klassen Hsp70 hos jäst som krävs för att upprätthålla en normal livslängd. Bilder av tvådimensionella proteingeler med proteinextrakt från vildtyp och Hsp70-mutanta jäststammar i mitten av den exponentiella tillväxtfasen. Jästen har odlats i rikt medium (YP) med 2 % glukos som kolkälla. Datasetet insamlades via fotografering av tvådimensionella proteingeler. Bildfilerna är i TIFF-format. 2012-06-13 2013-04-10 CellsCells CellerCeller Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (bagarjäst) Still image Total universe/Complete enumerationTotal universe/Complete enumeration Hela populationen/total räkningHela populationen/total räkning Biological testsBiological tests Biologiska provtagningarBiologiska provtagningar Access to data through SND. Data are freely accessible. Åtkomst till data via SND. Data är fritt tillgängliga. openAccess Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, Johansson G, Gustafsson T, Kvint K, Nyström T. Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. bioRxiv. 2020 Jun 29. Available from: https://www.biorxiv.org/content/10.1101/2020.06.25.170670v2.full Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, Johansson G, Gustafsson T, Kvint K, Nyström T. Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. bioRxiv. 2020 Jun 29. Available from: https://www.biorxiv.org/content/10.1101/2020.06.25.170670v2.full 10.1101/2020.06.25.170670 2020 Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, et al. (2021) Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. PLoS Genet 17(1): e1008951. Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, et al. (2021) Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. PLoS Genet 17(1): e1008951. 10.1371/journal.pgen.1008951 2021