Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Silver stained 2D-gels of protein extracts from wild type and Hsp70-mutant yeast strains
type: Dataset
description: Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts. Images of two dimensional protein gels with protein extracts from wild type and Hsp70 mutant yeast strains in mid-exponential growth phase. The yeast were grown in rich medium (YP) with 2 % glucose as carbon source. The images have been aligned and the background has been removed. The dataset was collected through imaging of two dimensional protein gels. The image files are provided in TIFF format.
citation: CreativeWork
  name: Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, Johansson G, Gustafsson T, Kvint K, Nyström T. Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. bioRxiv. 2020 Jun 29. Available from: https://www.biorxiv.org/content/10.1101/2020.06.25.170670v2.full
citation: CreativeWork
  name: Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, et al. (2021) Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. PLoS Genet 17(1): e1008951.
conditions of access: http://publications.europa.eu/resource/authority/access-right/PUBLIC
conditions of access: info:eu-repo/semantics/openAccess
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: David Öling
creator: Rebecca Andersson
creator: Thomas Nyström
date modified: 2021-02-11
date published: 2020-12-16
funding: Grant
  funder: Knut and Alice Wallenberg Foundation
language: English
is part of: https://doi.org/10.5878/nhpb-d307
keywords: Cell Biology
  type: DefinedTerm
  term code: 10604
keywords: Microbiology
  type: DefinedTerm
  term code: 10606
keywords: Cell and Molecular Biology
  type: DefinedTerm
  term code: 30108
keywords: Molecular Biology
  type: DefinedTerm
  term code: 10616
keywords: Cellular Senescence
keywords: Molecular Chaperones
keywords: HSP70 Heat-Shock Proteins
keywords: Proteostasis Deficiencies
license: https://creativecommons.org/licenses/by/4.0/
publisher: University of Gothenburg
temporal coverage: 2012/2020
distribution: data/2d gel electrophoresis/2D gel electrophoresis.zip
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version: 1

PropertyValue
property ID: DOI
value: 10.5878/9xxe-g981

PropertyValue
property ID: DOI
value: 10.1101/2020.06.25.170670

PropertyValue
property ID: DOI
value: 10.1371/journal.pgen.1008951

David Öling
type: Person
affiliation: AstraZeneca, Mölnlycke, Sweden
  type: Organization
  department: Discovery Biology, Discovery Sciences, Innovative Medicines and Early Development Biotech Unit
family name: Öling
given name: David

Rebecca Andersson
type: Person
affiliation: University of Gothenburg
family name: Andersson
given name: Rebecca

University of Gothenburg
type: Organization
department: Institute of Biomedicine, Department of Microbiology and Immunology

Thomas Nyström
type: Person
affiliation: University of Gothenburg
family name: Nyström
given name: Thomas

Knut and Alice Wallenberg Foundation
type: Organization

English
type: Language
alternate name: eng

Cellular Senescence
type: DefinedTerm
description: Process by which cells irreversibly stop dividing and enter a state of permanent growth arrest without undergoing CELL DEATH. Senescence can be induced by DNA DAMAGE or other cellular stresses, such as OXIDATIVE STRESS.
term code: D016922

Molecular Chaperones
type: DefinedTerm
description: A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
term code: D018832

HSP70 Heat-Shock Proteins
type: DefinedTerm
description: A class of MOLECULAR CHAPERONES found in both prokaryotes and in several compartments of eukaryotic cells. These proteins can interact with polypeptides during a variety of assembly processes in such a way as to prevent the formation of nonfunctional structures.
term code: D018840

Proteostasis Deficiencies
type: DefinedTerm
description: Disorders caused by imbalances in the PROTEIN HOMEOSTASIS network - synthesis, folding, and transport of proteins; post-translational modifications; and degradation or clearance of misfolded proteins.
term code: D057165