Prokaryotic plankton respiration, growth and abundance in the Arctic Ocean 2021
type: Dataset
description: The dataset consists of results from measurements of prokaryotic (Bacteria and Archaea) plankton respiration, growth, and abundance in the Arctic Ocean surface (0-500 m) in 2021. Data was collected during the Synoptic Arctic Survey expedition with the Swedish research icebreaker Oden between July 25 and September 20, 2021. Samples were collected primarily with Niskin-bottles on a rosette-sampler equipped with a CTD probe. Some samples were also taken in the boundary layer between ice and seawater with a Ruttner sampler after a hole to the water surface was drilled. Process speeds were measured on board within an hour of sampling in a cool laboratory with a temperature of 7°C. Plankton respiration was measured by oxygen optodes in eight simultaneous samples incubated at in situ temperature. For the PROMAC project, all samples were pre-filtered with 1.2 μm filters to specifically measure prokaryotic respiration. Growth of the prokaryotic community was measured by uptake of the DNA base thymidine labeled with tritium incubated at in situ temperature. The presence of prokaryotes was measured by epifluorescence microscopy after labeling with the dye acridine orange. Cell morphology and interactions were also studied via scanning electron microscopy in samples preserved with glutaraldehyde. In the filtered samples, the presence of different prokaryotic taxa was determined by sequencing the 16S RNA gene. In addition, expression of genes from isolated mRNA was measured. For metagenomics and transcriptomics from unfiltered samples, please refer to the OMICS project from the same expedition. The main projects (“SASIce24h” and “SAS”) aim to contribute knowledge about prokaryotic plankton respiration, growth rate and total biomass to better understand the turnover of carbon in the Arctic Ocean and its regulation. The results from the expedition will also provide a basis for comparison with similar studies in the future to follow climate change. The spatial coverage will increas…
conditions of access: http://publications.europa.eu/resource/authority/access-right/PUBLIC
conditions of access: info:eu-repo/semantics/openAccess
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: Johan Wikner
creator: Jarone Pinhassi
creator: Ashish Verma
creator: Dennis Amnebrink
date modified: 2024-01-16
date published: 2024-01-16
funding: Morfologisk och genetisk anpassning hos Arktiska bakterier vid extremt låg tillväxt
  type: Grant
  funder: The Kempe foundations
language: English
is part of: https://doi.org/10.5878/dgth-6q27
keywords: Climate Science
  type: DefinedTerm
  term code: 10501
keywords: Environmental Sciences
  type: DefinedTerm
  term code: 10502
keywords: Microbiology
  type: DefinedTerm
  term code: 10606
keywords: Ecology
  type: DefinedTerm
  description: Including: Biodiversity Conservation
  term code: 10611
keywords: Biota
keywords: Climatology / Meteorology / Atmosphere
keywords: Oceans
keywords: Environment
keywords: procaryote
keywords: Habitats and biotopes
keywords: Sea regions
keywords: arctic research
keywords: marine ecology
keywords: arctic region
keywords: microbial ecology
keywords: prokaryotes
  type: DefinedTerm
  term code: 50118
keywords: archaea
keywords: bacteria cavalier-smith, 2002
license: https://creativecommons.org/licenses/by/4.0/
publisher: Umeå University
spatial coverage: Arctic Ocean
temporal coverage: 2021-07-25/2021-09-20
distribution: data/SO21_WP5_ASAP_primarydata_240112.xlsx
  type: DataDownload
  additional type: data
  conditions of access: http://publications.europa.eu/resource/authority/access-right/PUBLIC
  conditions of access: info:eu-repo/semantics/openAccess
  content size: 359755
  encoding format: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
distribution: documentation/README SO21_WP5_ASAP_primarydata.pdf
  type: DataDownload
  additional type: documentation
  conditions of access: http://publications.europa.eu/resource/authority/access-right/PUBLIC
  conditions of access: info:eu-repo/semantics/openAccess
  content size: 329522
  encoding format: application/pdf
version: 1

PropertyValue
property ID: DOI
value: 10.5878/6qa4-cn46

principalRegistrationNumber
type: PropertyValue
property ID: https://www.rikstermbanken.se/termposter/1848/diarienummer
value: SMK-1851

Johan Wikner
type: Person
affiliation: Umeå University
family name: Wikner
given name: Johan

Umeå University
type: Organization
department: Ecology and Environmental Science

Jarone Pinhassi
type: Person
affiliation: Linneaus University
  type: Organization
  department: Biology and Environmental science
family name: Pinhassi
given name: Jarone

Ashish Verma
type: Person
affiliation: Umeå University
family name: Verma
given name: Ashish

Dennis Amnebrink
type: Person
affiliation: Linneaus University
family name: Amnebrink
given name: Dennis

Linneaus University
type: Organization
department: Biology and Environmental Scinece

The Kempe foundations
type: Organization

English
type: Language
alternate name: eng

Biota
type: DefinedTerm
description: Flora and/or fauna in natural environment.
term code: biota

Climatology / Meteorology / Atmosphere
type: DefinedTerm
description: Processes and phenomena of the atmosphere.
term code: climatology-meteorology-atmosphere

Oceans
type: DefinedTerm
description: Features and characteristics of salt water bodies (excluding inland waters).
term code: oceans

Environment
type: DefinedTerm
description: Environmental resources, protection and conservation.
term code: environment

procaryote
type: DefinedTerm
description: Organisms (i.e. prokaryotes) whose genetic material (filaments of DNA) is not enclosed by a nuclear membrane, and that do not possess mitochondria or plastids. Bacteria and cyanophyta are the only prokaryotic organisms.
term code: 6646

Habitats and biotopes
type: DefinedTerm
description: Geographical areas characterised by specific ecological conditions, processes, structure, and (life support) functions that physically support the organisms that live there. Includes terrestrial and aquatic areas distinguished by geographical, abiotic and biotic features, whether entirely natural or semi-natural.
term code: hb

Sea regions
type: DefinedTerm
description: Physical conditions of seas and saline water bodies divided into regions and sub-regions with common characteristics.
term code: sr

arctic research
type: DefinedTerm
term code: p12194

marine ecology
type: DefinedTerm
term code: p15041

arctic region
type: DefinedTerm
term code: p12434

microbial ecology
type: DefinedTerm
term code: p16314

archaea
type: DefinedTerm
term code: R

bacteria cavalier-smith, 2002
type: DefinedTerm
term code: B