10X single-cell RNA sequencing of hematopoietic stem and progenitor cells from a dual SF3B1-mutant MDS-RS patient and a healthy donor 10X single-cell RNA sequencing of hematopoietic stem and progenitor cells from a dual SF3B1-mutant MDS-RS patient and a healthy donor 2023-222-1 https://doi.org/10.48723/tt0e-eq82 Swedish National Data Service Svensk nationell datatjänst Landing page 10X single-cell RNA sequencing of hematopoietic stem and progenitor cells from a dual SF3B1-mutant MDS-RS patient and a healthy donor 10X single-cell RNA sequencing of hematopoietic stem and progenitor cells from a dual SF3B1-mutant MDS-RS patient and a healthy donor 2023-222-1 https://doi.org/10.48723/tt0e-eq82 10.1016/j.bneo.2024.100011 Moura, Pedro Luis Moura, Pedro Luis Hellström-Lindberg, Eva Hellström-Lindberg, Eva 19 0200 19 0200 2017.0359 2017.0359 2021-01404_VR 2021-01404_VR 21 0340 21 0340 Swedish National Data Service Svensk nationell datatjänst Landing page This dataset consists of 10X single-cell RNA sequencing data of bone marrow cells (CD34+ stem cells) obtained from two clinical visits of a dual SF3B1-mutant MDS-RS patient (Patient 1 in the associated publication: visits at 31 months post-diagnosis, with dominant SF3B1 N626D; and at 118 months post-diagnosis, with dominant SF3B1 K666N) and one healthy bone marrow donor. The objective of this data collection was to assess how the hematopoietic stem and progenitor cell compartment of this patient differed over time and as a result of changes in clonal frequency. The dataset is appoximately 80.9 GB and has filetypes txt, rds and fastq. Syftet med denna datainsamling var att bedöma hur det hematopoetiska stam- och progenitorcellsutrymmet hos en patient skiljde sig över tiden och som ett resultat av förändringar i klonal frekvens. Se den engelska beskrivningen för mer information. CellsCells CellerCeller Patients with Myelodysplastic neoplasms with ring sideroblasts (MDS-RS) and healthy donors Patienter med myelodysplastisk syndrom med ringsideroblaster (MDS-RS) samt friska donatorer Numeric Other Time series: DiscreteTime series: Discrete Tidsserie: DiskretTidsserie: Diskret The data in this dataset comes from 1 dual SF3B1mt MDS-RS patient and 1 healthy donor. It is part of a study where bone marrow (BM) samples were collected from 2 dual SF3B1mt MDS-RS patients (Patient 1: N626D, K666N; Patient 2: K700E, K666N) evaluated at Karolinska University Hospital, Sweden. Diagnostic procedures were performed according to the European LeukemiaNet recommendation and WHO classification for myeloid neoplasms. Mutational status was evaluated at the clinic through panel sequencing for the most common myeloid mutations. Additional samples were collected from a total of 4 healthy NBM donors for control purposes. All source material was provided with written informed consent for research use, given in accordance with the Declaration of Helsinki, and the study was approved by the Ethics Research Committee at Karolinska Institutet.The data in this dataset comes from 1 dual SF3B1mt MDS-RS patient and 1 healthy donor. It is part of a study where bone marrow (BM) samples were collected from 2 dual SF3B1mt MDS-RS patients (Patient 1: N626D, K666N; Patient 2: K700E, K666N) evaluated at Karolinska University Hospital, Sweden. Diagnostic procedures were performed according to the European LeukemiaNet recommendation and WHO classification for myeloid neoplasms. Mutational status was evaluated at the clinic through panel sequencing for the most common myeloid mutations. Additional samples were collected from a total of 4 healthy NBM donors for control purposes. All source material was provided with written informed consent for research use, given in accordance with the Declaration of Helsinki, and the study was approved by the Ethics Research Committee at Karolinska Institutet. Probability: Simple randomProbability: Simple random Sannolikhetsurval: obundet slumpmässigt urvalSannolikhetsurval: obundet slumpmässigt urval Non-probability: AvailabilityNon-probability: Availability Icke-sannolikhetsurval: tillgänglighetsurvalIcke-sannolikhetsurval: tillgänglighetsurval Mixed probability and non-probabilityMixed probability and non-probability Blandat sannolikhets- och icke-sannolikhetsurvalBlandat sannolikhets- och icke-sannolikhetsurval Access to data through SND. Access to data is restricted. Åtkomst till data via SND. Tillgång till data är begränsad. restrictedAccess Pedro Luis Moura, Yasuhito Nannya, Affaf Aliouat, Isabel Juliana Hofman, Teresa Mortera Blanco, Tetsuichi Yoshizato, Ryunosuke Saiki, Masahiro M Nakagawa, Maria Creignou, Ann-Charlotte Björklund, Gunilla Walldin, Indira Barbosa, Monika Jansson, Francesca Grasso, Edda M Elvarsdottir, Petter S Woll, Sten Eirik W Jacobsen, Seishi Ogawa, Eva Hellström-Lindberg, Competition of dual SF3B1mt clones in MDS-RS is associated with distinct RNA mis-splicing in hematopoietic stem cells, Blood Neoplasia, 2024,100011, ISSN 2950-3280, https://doi.org/10.1016/j.bneo.2024.100011. Pedro Luis Moura, Yasuhito Nannya, Affaf Aliouat, Isabel Juliana Hofman, Teresa Mortera Blanco, Tetsuichi Yoshizato, Ryunosuke Saiki, Masahiro M Nakagawa, Maria Creignou, Ann-Charlotte Björklund, Gunilla Walldin, Indira Barbosa, Monika Jansson, Francesca Grasso, Edda M Elvarsdottir, Petter S Woll, Sten Eirik W Jacobsen, Seishi Ogawa, Eva Hellström-Lindberg, Competition of dual SF3B1mt clones in MDS-RS is associated with distinct RNA mis-splicing in hematopoietic stem cells, Blood Neoplasia, 2024,100011, ISSN 2950-3280, https://doi.org/10.1016/j.bneo.2024.100011. 10.1016/j.bneo.2024.100011 2024