Detection of multiple fish species in the diet of the invasive round goby reveals new trophic interactions in the Baltic Sea
type: Dataset
description: The purpose of the study was to investigate round goby diet in two geographically distant areas in the Baltic Sea in two consecutive years, using two different methods. We collected round goby using fyke nets and gill nets in May and June 2018-2019 in Karlskrona in the southern Baltic Sea and on Åland in the northern Baltic Sea. The data set contains data of round goby diet, collected through both visual stomach content analysis (VSCA) and DNA metabarcoding. In VSCA, we dissected round goby and determined the prey items to the lowest possible taxonomic level, counted the individual prey/prey groups and estimated their relative proportions in each sample. In DNA metabarcoding, we used the 12S marker for detection of fish species in round goby diet, and the COI marker for an estimation of the proportions of fishes vs. invertebrates in the diet. DNA from round goby stomach samples was amplified using PCR and sequenced on the Illumina MiSeq platform. The output is then provided as number of sequences per prey species per sample, which roughly corresponds to relative biomass per prey species per sample. This makes it possible to compare approximate diet proportions between VSCA and DNA metabarcoding. We then analyzed round goby diet variation between areas and years for VSCA and metabarcoding (12S) separately with Redundancy analysis (RDA). In order to investigate how round goby environmental abundances potentially influenced round goby diet, we analyzed how the fyke net catches varied between areas, years and months. The data of round goby environmental abundances was standardized for catch per unit effort (CPUE) to make comparisons possible. We then related the fyke net catches to number of prey aggregated in prey groups in round goby diet using linear mixed models (LMM) and general linear mixed models (GLMM). To minimize the risk of drawing the wrong conclusions from the analysis of how round goby environmental abundances potentially influenced round goby diet, we als…
citation: CreativeWork
  name: Wallin Kihlberg I, Florin A-B, Lundström K, Östman Ö (2023) Detection of multiple fish species in the diet of the invasive round goby reveals new trophic interactions in the Baltic Sea. Aquatic Invasions 18(2): 141–162.
conditions of access: http://publications.europa.eu/resource/authority/access-right/PUBLIC
conditions of access: info:eu-repo/semantics/openAccess
contributor: Karl Lundström
contributor: Örjan Östman
contributor: Ann-Britt Florin
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: Isa Wallin Kihlberg
date modified: 2023-07-03
date published: 2023-04-28
funding: Svartmunnad smörbult i Blekinge och Kalmar län: utbredning, spridning, påverkan på ekosystem och potential som resurs
  type: Grant
  identifier: Jnr 2016-7109
  funder: Swedish Board of Agriculture
funding: DNA-metabarcoding av diet hos den invasiva svartmunnade smörbulten
  type: Grant
  identifier: Hav Dnr 3484-21
  funder: Swedish Agency for Marine and Water Management
funding: Ekologiska effekter av den invasiva fisken svartmunnad smörbult i Östersjön
  type: Grant
  identifier: FO 2017-0047
  funder: Oscar and Lili Lamm memorial foundation
language: English
is part of: https://doi.org/10.5878/hm8w-b112
keywords: Ecology
  type: DefinedTerm
  description: Including: Biodiversity Conservation
  term code: 10611
keywords: Round goby
keywords: Neogobius melanostomus
license: https://creativecommons.org/licenses/by/4.0/
publisher: Swedish University of Agricultural Sciences
spatial coverage: Blekinge County
spatial coverage: Åland Islands
spatial coverage: Baltic Sea
temporal coverage: 2018-05-01/2018-06-30
temporal coverage: 2019-05-01/2019-06-30
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Karl Lundström
type: Person
affiliation: Swedish University of Agricultural Sciences
family name: Lundström
given name: Karl

Swedish University of Agricultural Sciences
type: Organization
department: Department of Aquatic Resources

Örjan Östman
type: Person
affiliation: Swedish University of Agricultural Sciences
family name: Östman
given name: Örjan

Ann-Britt Florin
type: Person
affiliation: Swedish University of Agricultural Sciences
family name: Florin
given name: Ann-Britt

Isa Wallin Kihlberg
type: Person
affiliation: Swedish University of Agricultural Sciences
family name: Wallin Kihlberg
given name: Isa

Swedish Board of Agriculture
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Swedish Agency for Marine and Water Management
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Oscar and Lili Lamm memorial foundation
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Blekinge County
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Åland Islands
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