RNA-sekvenseringsdata från patienter med hjärtsjukdom och kontroller RNA sequencing data from patients with heart disease and controls 2023-80-1-1 https://doi.org/10.5878/e48r-gn02 Swedish National Data Service Svensk nationell datatjänst Landing page RNA-sekvenseringsdata från patienter med hjärtsjukdom och kontroller RNA sequencing data from patients with heart disease and controls 2023-80-1-1 https://doi.org/10.5878/e48r-gn02 10.1016/j.ijcard.2021.09.046 Rotter Sopasakis, Victoria Rotter Sopasakis, Victoria Mattsson Hultén, Lillemor Mattsson Hultén, Lillemor Swedish National Data Service Svensk nationell datatjänst Landing page Heart Diseases Hjärtsjukdomar Aortic Valve Aortaklaff Coronary Artery Bypass Koronar bypass Cadherins Kadheriner Heart Valve Prosthesis Implantation Hjärtklaffprotesimplantation RNA, Long Noncoding Långt icke-kodande RNA Cadherin Related Proteins Kadherinrelaterade proteiner RNA sequencing analysis of atrial cardiac tissue from patients undergoing coronary artery bypass grafting (CABG) or aortic valve replacement (AVR) was performed and compared with atrial cardiac tissue from organ donors and purchased human atrial cardiac RNA. RNA sequencing analysis was performed at the Genomics Core Facility at University of Gothenburg, Sweden. All samples (ten controls, ten CABG patients and ten AVR patients) were quality checked by the RNA integrity number (RIN) using Tapestation 2200 RNA screenTape (Agilent Technologies, Santa Clara, CA) and RNA concentration was measured by NanoDrop (Thermo Fisher, Waltham, MA). RIN values ranged between 6.6 and 9.0 for all samples. TruSeq Total Stranded RNA kit with RiboZero (Gold) Sample Preparation Guide (15,031,048 Rev. E) was used for RNA sample preparation (Illumina, San Diego, CA). A total of 10 μl (~1 μg) RNA from each sample was used for library preparation. Directly after depletion, a cleanup step was performed using 110 ul of the RNAClean XP beads (Beckman Coulter, USA) for each sample. The fragmentation step was performed for 8 min. 12 PCR cycles were run for all samples. Libraries were quantified and normalized with Qubit DNA HS Assay kit (Life Technologies, Carlsbad, CA) and fragment size determined by Tapestation 2200 (Agilent Technologies, Santa Clara, CA). The libraries were pooled together by using the Illumina protocol for pooling and sequenced with NovaSeq 6000 S1 (Illumina, San Diego, CA) for the read length of 2 × 100 bp. Data i denna studie kommer från RNA-sekvenseringsanalys av förmaksvävnad från humana hjärtan från patienter som genomgick coronary artery bypass grafting (CABG) eller aortic valve replacement (AVR) samt förmaksvävnad från organdonatorer (kontroller) och inköpt humant RNA från förmaksvävnad (kontroller). Sweden Sverige IndividualIndividual IndividIndivid Patients undergoing coronary artery bypass grafting (CABG) or aortic valve replacement (AVR) (experimental group) and organ donors and purchased human RNA (control group). Patienter som genomgått coronary artery bypass grafting (CABG) or aortic valve replacement (AVR) (experimentell grupp) och organdonatorer och inköpt humant RNA (kontrollgrupp). Numeric Non-probability: AvailabilityNon-probability: Availability Icke-sannolikhetsurval: tillgänglighetsurvalIcke-sannolikhetsurval: tillgänglighetsurval Access to data through SND. Access to data is restricted. Åtkomst till data via SND. Tillgång till data är begränsad. restrictedAccess Joakim Sandstedt, Kristina Vukusic, Elham Rekabdar, Göran Dellgren, Anders Jeppsson, Lillemor Mattsson Hultén, Victoria Rotter Sopasakis. Markedly reduced myocardial expression of γ-protocadherins and long non-coding RNAs in patients with heart disease. International Journal of Cardiology, vol 344 (2021), page 149–159. Joakim Sandstedt, Kristina Vukusic, Elham Rekabdar, Göran Dellgren, Anders Jeppsson, Lillemor Mattsson Hultén, Victoria Rotter Sopasakis. Markedly reduced myocardial expression of γ-protocadherins and long non-coding RNAs in patients with heart disease. International Journal of Cardiology, vol 344 (2021), page 149–159. 10.1016/j.ijcard.2021.09.046 2021