urn:ddi:se.researchdata:2024-225:1 SND 2024-225 1 urn:ddi:se.researchdata:2024-225.ResourcePackage:2.0 urn:ddi:se.researchdata:2024-225.OtherMaterialScheme:2.0 urn:ddi:se.researchdata:2024-225.OrganizationScheme-0:2.0 urn:ddi:se.researchdata:2024-225.Individual-0:2.0 affiliation Linköping University Robin N Abbey-Lee Robin N Abbey-Lee urn:ddi:se.researchdata:2024-225.Individual-0:2.0 affiliation Department of Zoology, Stockholm University Emily J Uhrig Emily J Uhrig urn:ddi:se.researchdata:2024-225.Individual-0:2.0 affiliation Institutionen för fysik, kemi och biologi, Linköping University Josefina Zidar Josefina Zidar urn:ddi:se.researchdata:2024-225.Individual-0:2.0 affiliation Department of Zoology, Stockholm University Anna Favati Anna Favati urn:ddi:se.researchdata:2024-225.Individual-0:2.0 affiliation Institutionen för fysik, kemi och biologi,, Linköping University Johan Almberg Johan Almberg urn:ddi:se.researchdata:2024-225.Individual-0:2.0 affiliation Department of Neuroscience, Uppsala University Josefin Dahlblom Josefin Dahlblom urn:ddi:se.researchdata:2024-225.Individual-0:2.0 affiliation Department of Neuroscience, Uppsala University Svante Winberg Svante Winberg urn:ddi:se.researchdata:2024-225.Individual-0:2.0 affiliation Institutionen för fysik, kemi och biologi, Linköpings University Hanne Løvlie Hanne Løvlie ORCID 0000-0003-4352-6275 urn:ddi:se.researchdata:2024-225.StudyUnit:2.0 2024-225 The influence of rearing on behavior, brain monoamines and gene expression in three-spined sticklebacks The influence of rearing on behavior, brain monoamines and gene expression in three-spined sticklebacks urn:ddi:se.researchdata:2024-225.Individual-0:2.0 Individual Linköpings universitet Linköping University Linköpings universitet Linköping University 2018-05-24 10.5878/1txx-m695 DOI Data file for the study: The influence of rearing on behavior, brain monoamines, and gene expression in three-spined sticklebacks, RN Abbey-Lee, EJ Uhrig, J Zidar, A Favati, J Almberg, J Dahlblom, S Winberg, H Lovlie. Corresponding Author: H Lovlie, hanne.lovlie@liu.se File contents: All data used for the study- including treatment status, behaviour, qPCR gene expression, and HPLC-EC monoamine levels. The causes of individual variation in behavior are often not well understood, and potential underlying mechanisms include both intrinsic and extrinsic factors, such as early environmental, physiological, and genetic differences. In an exploratory laboratory study, we raised three-spined sticklebacks (Gasterosteus aculeatus) under 4 different environmental conditions (simulated predator environment, complex environment, variable social environment, and control). We investigated how these manipulations related to behavior, brain physiology and gene expression later in life, with focus on brain dopamine and serotonin levels, turnover rates, and gene expression. The different rearing environments influenced behavior and gene expression, but did not alter monoamine levels or metabolites. Specifically, compared to control fish, fish exposed to a simulated predator environment tended to be less aggressive, more exploratory, and more neophobic; and fish raised in both complex and variable social environments tended to be less neophobic. Exposure to a simulated predator environment tended to lower expression of dopamine receptor DRD4A, a complex environment increased expression of dopamine receptor DRD1B, while a variable social environment tended to increase serotonin receptor 5-HTR2B and increased serotonin transporter SLC6A4A expression. Despite both behavior and gene expression varying with early environment, there was no evidence that gene expression mediated the relationship between early environment and behavior. Our results confirm that environmental conditions early in life can affect phenotypic variation. However, the mechanistic pathway of the monoaminergic systems translating early environmental variation into observed behavioral responses was not detected. Datasetet har ursprungligen publicerats i DiVA och flyttades över till SND 2024. Data file for the study: The influence of rearing on behavior, brain monoamines, and gene expression in three-spined sticklebacks, RN Abbey-Lee, EJ Uhrig, J Zidar, A Favati, J Almberg, J Dahlblom, S Winberg, H Lovlie. Corresponding Author: H Lovlie, hanne.lovlie@liu.se File contents: All data used for the study- including treatment status, behaviour, qPCR gene expression, and HPLC-EC monoamine levels. The causes of individual variation in behavior are often not well understood, and potential underlying mechanisms include both intrinsic and extrinsic factors, such as early environmental, physiological, and genetic differences. In an exploratory laboratory study, we raised three-spined sticklebacks (Gasterosteus aculeatus) under 4 different environmental conditions (simulated predator environment, complex environment, variable social environment, and control). We investigated how these manipulations related to behavior, brain physiology and gene expression later in life, with focus on brain dopamine and serotonin levels, turnover rates, and gene expression. The different rearing environments influenced behavior and gene expression, but did not alter monoamine levels or metabolites. Specifically, compared to control fish, fish exposed to a simulated predator environment tended to be less aggressive, more exploratory, and more neophobic; and fish raised in both complex and variable social environments tended to be less neophobic. Exposure to a simulated predator environment tended to lower expression of dopamine receptor DRD4A, a complex environment increased expression of dopamine receptor DRD1B, while a variable social environment tended to increase serotonin receptor 5-HTR2B and increased serotonin transporter SLC6A4A expression. Despite both behavior and gene expression varying with early environment, there was no evidence that gene expression mediated the relationship between early environment and behavior. Our results confirm that environmental conditions early in life can affect phenotypic variation. However, the mechanistic pathway of the monoaminergic systems translating early environmental variation into observed behavioral responses was not detected. urn:ddi:se.researchdata:2024-225.TopicalCoverage:2.0 Biological Sciences Biologi Brain Mapping Hjärnans kartläggning Gene Expression Genuttryck urn:ddi:se.researchdata:2024-225.Archive:2.0 urn:ddi:se.researchdata:2024-225.Archive-ArchiveSpecificType-AccessType:2.0 openAccess 1