Treatment with (R)-α-methylhistamine or IL4 stimulates mucin production and decreases Helicobacter pylori density in the murine stomach Treatment with RαMH or IL4 stimulates mucin production and decreases Helicobacter pylori density in the murine stomach Treatment with (R)-α-methylhistamine or IL4 stimulates mucin production and decreases Helicobacter pylori density in the murine stomach 2024-467-1 https://doi.org/10.5878/hg1a-sd78 Swedish National Data Service Svensk nationell datatjänst Landing page Treatment with (R)-α-methylhistamine or IL4 stimulates mucin production and decreases Helicobacter pylori density in the murine stomach Treatment with RαMH or IL4 stimulates mucin production and decreases Helicobacter pylori density in the murine stomach Treatment with (R)-α-methylhistamine or IL4 stimulates mucin production and decreases Helicobacter pylori density in the murine stomach 2024-467-1 https://doi.org/10.5878/hg1a-sd78 10.1080/21505594.2019.1573050 10.1128/IAI.01000-12 10.1023/a:1018841204104 Santos, Licínia Santos, Licínia Sharba, Sinan Sharba, Sinan Benktander, John Benktander, John Ojaimi Loibman, Stefany Ojaimi Loibman, Stefany Quintana-Hayashi, Macarena P. Quintana-Hayashi, Macarena P. Erhardsson, Mattias Erhardsson, Mattias Lindén, Sara K. Lindén, Sara K. Swedish National Data Service Svensk nationell datatjänst Landing page Mucins Muciner Mucus Slem Stomach Magsäck HEALTH HÄLSA Medication and treatment Medicinering och behandlingar The histology data show the sum scores for each group used to calculate the results in percentages normalized to non-infected vehicle-treated or H. pylori-infected vehicle-treated mice allowing for pooling results from different experiments into the same graph. The antibody levels determined by ELISA are presented in absorbance 450nm. The cell growth/viability was measured as the reducing potential of viable cell data presented in luminescence. The expression of virulence factor assessed by RT-PCR is expressed in fold-change. Histologiska data visar summapoängen för varje grupp som användes för att beräkna resultaten i procentandelar normaliserade till icke-infekterade vehikelbehandlade eller H. pylori-infekterade vehikelbehandlade möss, vilket möjliggör sammanslagning av resultat från olika experiment i samma graf. Antikroppsnivåerna bestämda med ELISA presenteras i absorbans 450 nm. Celltillväxten/viabiliteten mättes som den reducerande potentialen för viabla celldata presenterade i luminescens. Uttrycket av virulensfaktor bedömd med RT-PCR uttrycks i veck-förändring. 2021-04-22 2021-05-06 2021-11-17 2021-12-02 2022-03-24 2022-04-09 2022-09-07 2022-09-22 OtherOther ÖvrigtÖvrigt CellsCells CellerCeller OrganOrgan OrganOrgan Mice were divided into different groups: non-infected vehicle-treated control, H. pylori vehicle-treated, H. pylori-infected RaMH-treated and H. pylori-infected Il4-treated. Möss delades in i olika grupper: icke-infekterad vehikelbehandlad kontroll, H. pylori-vehikelbehandlad, H. pylori-infekterad RaMH-behandlad och H. pylori-infekterad Il4-behandlad. Numeric Text OtherOther ÖvrigtÖvrigt A total of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO and diluted in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.A total of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO and diluted in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use. Totalt 300 µl per mus framställdes genom att lösa 2,6 mg GalNAz i 50 µl DMSO och späddes i PBS. GalNAz-injektionerna gavs intraperitonealt 14 dagar efter infektion, och möss avlivades 2 timmar efter injektionen genom cervikal dislokation under anestesi med isofluran. Helblodsprover från varje mus samlades in vid avlivning. Därefter skördades magarna och öppnades längs den större krökningen följt av en försiktig tvätt i steril PBS för att avlägsna chyme. En remsa av magsäckens mindre krökning innehållande förmage, corpus och antrum fixerades i buffrad formaldehyd 4% vattenlösning i 24 timmar. Den fixerade vävnaden var paraffininbäddad och histologiska objektglas preparerades med 4 µm tjocka vävnadsband. Dessutom samlades två delar av korpusen (ungefär 4 x 4 mm) upp. En placerades i RNA-later och lagrades vid 4°C, den andra frystes omedelbart i torris. Båda proverna förvarades senare vid -80°C fram till användning.Totalt 300 µl per mus framställdes genom att lösa 2,6 mg GalNAz i 50 µl DMSO och späddes i PBS. GalNAz-injektionerna gavs intraperitonealt 14 dagar efter infektion, och möss avlivades 2 timmar efter injektionen genom cervikal dislokation under anestesi med isofluran. Helblodsprover från varje mus samlades in vid avlivning. Därefter skördades magarna och öppnades längs den större krökningen följt av en försiktig tvätt i steril PBS för att avlägsna chyme. En remsa av magsäckens mindre krökning innehållande förmage, corpus och antrum fixerades i buffrad formaldehyd 4% vattenlösning i 24 timmar. Den fixerade vävnaden var paraffininbäddad och histologiska objektglas preparerades med 4 µm tjocka vävnadsband. Dessutom samlades två delar av korpusen (ungefär 4 x 4 mm) upp. En placerades i RNA-later och lagrades vid 4°C, den andra frystes omedelbart i torris. Båda proverna förvarades senare vid -80°C fram till användning. OtherOther ÖvrigtÖvrigt A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use. Measurements and testsMeasurements and tests Mätningar och testerMätningar och tester A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use. Measurements and testsMeasurements and tests Mätningar och testerMätningar och tester A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use. A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use. Access to data through SND. Data are freely accessible. Åtkomst till data via SND. Data är fritt tillgängliga. openAccess Interleukin 4 induces rapid mucin transport, increases mucus thickness and quality and decreases colitis and Citrobacter rodentium in contact with epithelial cells S. Sharba, N. Navabi, M. Padra, J. A. Persson, M. P. Quintana-Hayashi, J. K. Gustafsson, et al. Virulence 2019 Vol. 10 Issue 1 Pages 97-117 Interleukin 4 induces rapid mucin transport, increases mucus thickness and quality and decreases colitis and Citrobacter rodentium in contact with epithelial cells S. Sharba, N. Navabi, M. Padra, J. A. Persson, M. P. Quintana-Hayashi, J. K. Gustafsson, et al. Virulence 2019 Vol. 10 Issue 1 Pages 97-117 10.1080/21505594.2019.1573050 2019 Helicobacter pylori infection impairs the mucin production rate and turnover in the murine gastric mucosa. Navabi, M. E. Johansson, S. Raghavan and S. K. Linden Infect Immun 2013 Vol. 81 Issue 3 Pages 829-37 Helicobacter pylori infection impairs the mucin production rate and turnover in the murine gastric mucosa. Navabi, M. E. Johansson, S. Raghavan and S. K. Linden Infect Immun 2013 Vol. 81 Issue 3 Pages 829-37 10.1128/IAI.01000-12 2013 Histological effect of (R)-alpha-methylhistamine on ethanol damage in rat gastric mucosa: influence on mucus production. Morini, D. Grandi, M. L. Arcari, G. Galanti and G. Bertaccini Dig Dis Sci 1997 Vol. 42 Issue 5 Pages 1020-8 Histological effect of (R)-alpha-methylhistamine on ethanol damage in rat gastric mucosa: influence on mucus production. Morini, D. Grandi, M. L. Arcari, G. Galanti and G. Bertaccini Dig Dis Sci 1997 Vol. 42 Issue 5 Pages 1020-8 10.1023/a:1018841204104 1997