Data för: A. salmonicida infection inhibits rainbow trout gill mucin production Data for: A. salmonicida infection inhibits rainbow trout gill mucin production 2024-487-1 https://doi.org/10.5878/r0k2-qh43 Swedish National Data Service Svensk nationell datatjänst Landing page Data för: A. salmonicida infection inhibits rainbow trout gill mucin production Data for: A. salmonicida infection inhibits rainbow trout gill mucin production 2024-487-1 https://doi.org/10.5878/r0k2-qh43 10.1080/21505594.2019.1573050 10.3390/microorganisms8121871 10.1038/mi.2008.5 10.1016/j.fsi.2022.01.031 Sharba, Sinan Sharba, Sinan 2018-01419 2019-01152 2019 2019-372 Swedish National Data Service Svensk nationell datatjänst Landing page Fishes Fiskar Gills Gälar Mucins Muciner Mucus Slem The scoring system for both mucus production and histopathology is described in the research article "A. salmonicida infection inhibits rainbow trout gill mucin production" in the methods section. A. salmonicida (Aeromonas salmonicida). The variables examined in the data set are quantitative data and based on two main histological scoring systems. In tabs 2, 5 and 6 the scoring is based on how far an injected label is transported within the cell from its starting point (perinuclear; around the nucleus) over time or during insult (such as exposure to bacteria). The goblet cell is split into 4 regions from bottom to top of the mucus producing cell; perinuclear, mid cytoplasm, near apical cell surface and at apical cell surface. Each location/compartment received a score from 0-4 based on intensity of the label. This method is executed in vivo fish (tabs 2 and 5); where in tab 2 the label is injected at time 0 and collected at different time points following the injected label to study mucus production over time. In tab 5, a similar method is used, however, for this the fish were grouped into three different timepoints (8 h, 32 h and 7 days after injection of the label) and further subgrouped into non-immersed control fish and immersed fish in different concentrations of bacteria. Hence, allowing for investigation of mucus production over time and under stimulation. In in vitro fish gill cells (tab 6) grown on cell culture plates grouped in either non-infected controls, infected and cytokine-treated groups were analyzed similarly at 6 h after addition of label, added to the growth medium. In tab 4: Histological sections stained with Hematoxylin/Eosin were evaluated for pathology. For this we used the groups of fish investigated in tab 5 (above). On a general basis, the different categories of tissue changes (17 in total) were assigned to two groups; group 1 encompassed tissue changes thought to represent less negative impact on the gill function and/ or that are usually seen as readily reversible, while group 2 contained tissue changes that are thought to possess a greater influence on the gill function and/ or take longer time to heal. To reflect this expected influence on the gill function, categories of tissue changes of group 2 were given a higher score compared to categories of tissue changes of group 1 given the same relative distribution of tissue changes. In theory, the maximum gill histopathology score with this scoring model was 104, but in general terms, a score under 10 was regarded as mild changes, a score from 10 to under 20 as moderate changes, while a score above 20 was regarded as severe change. The data is available in excel-format. Datasetet innehåller provresultat från en studie av hur bakterien A. salmonicida påverkar gälar hos regnbåge (O. mykiss). Se den engelskspråkiga beskrivningen för ytterligare information. Sweden Sverige Numeric Text Access to data through SND. Data are freely accessible. Åtkomst till data via SND. Data är fritt tillgängliga. openAccess Sharba S, Navabi N, Padra M, Persson JA, Quintana-Hayashi MP, Gustafsson JK, Szeponik L, Venkatakrishnan V, Sjöling Å, Nilsson S, Quiding-Järbrink M, Johansson MEV, Linden SK. Interleukin 4 induces rapid mucin transport, increases mucus thickness and quality and decreases colitis and Citrobacter rodentium in contact with epithelial cells. Virulence. 2019 Dec;10(1):97-117. doi: 10.1080/21505594.2019.1573050. PMID: 30665337; PMCID: PMC6363059. Sharba S, Navabi N, Padra M, Persson JA, Quintana-Hayashi MP, Gustafsson JK, Szeponik L, Venkatakrishnan V, Sjöling Å, Nilsson S, Quiding-Järbrink M, Johansson MEV, Linden SK. Interleukin 4 induces rapid mucin transport, increases mucus thickness and quality and decreases colitis and Citrobacter rodentium in contact with epithelial cells. Virulence. 2019 Dec;10(1):97-117. doi: 10.1080/21505594.2019.1573050. PMID: 30665337; PMCID: PMC6363059. 10.1080/21505594.2019.1573050 2019 Benktander J, Padra JT, Maynard B, Birchenough G, Botwright NA, McCulloch R, Wynne JW, Sharba S, Sundell K, Sundh H, Lindén SK. Gill Mucus and Gill Mucin O-glycosylation in Healthy and Amebic Gill Disease-Affected Atlantic Salmon. Microorganisms. 2020 Nov 26;8(12):1871. doi: 10.3390/microorganisms8121871. PMID: 33256221; PMCID: PMC7768351. Benktander J, Padra JT, Maynard B, Birchenough G, Botwright NA, McCulloch R, Wynne JW, Sharba S, Sundell K, Sundh H, Lindén SK. Gill Mucus and Gill Mucin O-glycosylation in Healthy and Amebic Gill Disease-Affected Atlantic Salmon. Microorganisms. 2020 Nov 26;8(12):1871. doi: 10.3390/microorganisms8121871. PMID: 33256221; PMCID: PMC7768351. 10.3390/microorganisms8121871 2020 Linden, S., Sutton, P., Karlsson, N. et al. Mucins in the mucosal barrier to infection. Mucosal Immunol 1, 183–197 (2008). https://doi.org/10.1038/mi.2008.5 Linden, S., Sutton, P., Karlsson, N. et al. Mucins in the mucosal barrier to infection. Mucosal Immunol 1, 183–197 (2008). https://doi.org/10.1038/mi.2008.5 10.1038/mi.2008.5 2008 Sharba S, Sundh H, Sundell K, Benktander J, Santos L, Birchenough G, Lindén SK. Rainbow trout gastrointestinal mucus, mucin production, mucin glycosylation and response to lipopolysaccharide. Fish Shellfish Immunol. 2022 Mar;122:181-190. doi: 10.1016/j.fsi.2022.01.031. Epub 2022 Jan 22. PMID: 35077869. Sharba S, Sundh H, Sundell K, Benktander J, Santos L, Birchenough G, Lindén SK. Rainbow trout gastrointestinal mucus, mucin production, mucin glycosylation and response to lipopolysaccharide. Fish Shellfish Immunol. 2022 Mar;122:181-190. doi: 10.1016/j.fsi.2022.01.031. Epub 2022 Jan 22. PMID: 35077869. 10.1016/j.fsi.2022.01.031 2022