Raw scRNA-seq FASTQ-datafiler för: Nerve growth factor responsive elements modulate immune cell inflammation and are dysregulated in an Alzheimer's disease mouse model Raw scRNA-seq FASTQ data files for: Nerve growth factor responsive elements modulate immune cell inflammation and are dysregulated in an Alzheimer's disease mouse model 2026-128-1 https://doi.org/10.48723/d5mw-0122 Swedish National Data Service Svensk nationell datatjänst Landing page Raw scRNA-seq FASTQ-datafiler för: Nerve growth factor responsive elements modulate immune cell inflammation and are dysregulated in an Alzheimer's disease mouse model Raw scRNA-seq FASTQ data files for: Nerve growth factor responsive elements modulate immune cell inflammation and are dysregulated in an Alzheimer's disease mouse model 2026-128-1 https://doi.org/10.48723/d5mw-0122 Eriksdotter, Maria Eriksdotter, Maria Gera, Ruchi Gera, Ruchi Swedish National Data Service Svensk nationell datatjänst Landing page Inflammation Inflammation Nerve Growth Factors Nervtillväxtfaktorer Cholinergic Neurons Kolinerga neuroner Immune cell phenotyping fenotypbestämning av immuncell Specific diseases, disorders and medical conditions Specifika sjukdomar, störningar och medicinska tillstånd This dataset contains raw single-cell RNA sequencing (scRNA-seq) FASTQ files generated from splenocytes isolated from wild-type C57BL/6J mice (n = 2). Libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3′ Reagent Kit v3.1 and sequenced on an Illumina NextSeq 2000 platform. The dataset consists of paired-end raw sequencing files associated with a study titled "Nerve growth factor responsive elements modulate immune cell inflammation and are dysregulated in an Alzheimer's disease mouse model". The dataset consists of four (4) files: - RGAD_WT_S1_L001_R1_001.fastq.gz Forward reads for WT sample 1 - RGAD_WT_S1_L001_R2_001.fastq.gz Reverse reads for WT sample 1 - RGAD_WT1_S1_L001_R1_001.fastq.gz Forward reads for WT sample 2 - RGAD_WT1_S1_L001_R2_001.fastq.gz Reverse reads for WT sample 2 Datasetet innehåller raw FASTQ-filer från single-cell RNA-sekvensering (scRNA-seq) genererade från splenocyter isolerade från vildtyp C57BL/6J-möss (n = 2). Bibliotek preparerades med 10x Genomics Chromium Next GEM Single Cell 3′ Reagent Kit v3.1 och sekvenserades på en Illumina NextSeq 2000-plattform. Datasetet består av parade råa sekvenseringsfiler kopplade till en studie med titeln "Nerve growth factor responsive elements modulate immune cell inflammation and are dysregulated in an Alzheimer's disease mouse model". The dataset consists of four (4) files: - RGAD_WT_S1_L001_R1_001.fastq.gz Forward reads for WT sample 1 - RGAD_WT_S1_L001_R2_001.fastq.gz Reverse reads for WT sample 1 - RGAD_WT1_S1_L001_R1_001.fastq.gz Forward reads for WT sample 2 - RGAD_WT1_S1_L001_R2_001.fastq.gz Reverse reads for WT sample 2 AnimalAnimal DjurDjur Wild-type Mus musculus C57BL/6J laboratory mice; splenocyte-derived single-cell suspensions used for scRNA-seq analysis. Vild typ Mus musculus C57BL/6J laboratoriemöss; single-cell-suspensioner från splenocyter använda för scRNA-seq-analys. Other 2 biological samples2 biological samples 2 biologiska prover2 biologiska prover Mice were kept on a 12h light/dark cycle and food and water were available ad libitum. Mice were deeply anesthetized using isoflurane to collect spleen. Dissected spleens were processed to collect splenic immune cells. After isolation, the splenocytes were counted and the viability was measured with the Luna FL dual fluorescence cell counter with dual stain Acridine Orange/Propidium Iodide, with cell size gated between 1-60 μm (Logos Biosystems). Cell suspensions with viability of 85% were encapsulated using the Next Gem Single Cell 3′ Reagent kit v3.1. (Dual index) from 10x Genomics (cat: PN-100026), aiming to capture 5,000 cells. Libraries were prepared as per manufacturer’s instruction (protocol CG000315) and sequenced using the Illumina platform on a Next seq2000 P3 100 cycles flow cell, aiming for an average read depth of 40,000 reads/cell. The cell capture and library generation were performed at the Single Cell Core Facility of Flemingsberg Campus (SICOF) that receives funding from the Infrastructure Board at Karolinska Institutet.Mice were kept on a 12h light/dark cycle and food and water were available ad libitum. Mice were deeply anesthetized using isoflurane to collect spleen. Dissected spleens were processed to collect splenic immune cells. After isolation, the splenocytes were counted and the viability was measured with the Luna FL dual fluorescence cell counter with dual stain Acridine Orange/Propidium Iodide, with cell size gated between 1-60 μm (Logos Biosystems). Cell suspensions with viability of 85% were encapsulated using the Next Gem Single Cell 3′ Reagent kit v3.1. (Dual index) from 10x Genomics (cat: PN-100026), aiming to capture 5,000 cells. Libraries were prepared as per manufacturer’s instruction (protocol CG000315) and sequenced using the Illumina platform on a Next seq2000 P3 100 cycles flow cell, aiming for an average read depth of 40,000 reads/cell. The cell capture and library generation were performed at the Single Cell Core Facility of Flemingsberg Campus (SICOF) that receives funding from the Infrastructure Board at Karolinska Institutet. Mice were kept on a 12h light/dark cycle and food and water were available ad libitum. Mice were deeply anesthetized using isoflurane to collect spleen. Dissected spleens were processed to collect splenic immune cells. After isolation, the splenocytes were counted and the viability was measured with the Luna FL dual fluorescence cell counter with dual stain Acridine Orange/Propidium Iodide, with cell size gated between 1-60 μm (Logos Biosystems). Cell suspensions with viability of 85% were encapsulated using the Next Gem Single Cell 3′ Reagent kit v3.1. (Dual index) from 10x Genomics (cat: PN-100026), aiming to capture 5,000 cells. Libraries were prepared as per manufacturer’s instruction (protocol CG000315) and sequenced using the Illumina platform on a Next seq2000 P3 100 cycles flow cell, aiming for an average read depth of 40,000 reads/cell. The cell capture and library generation were performed at the Single Cell Core Facility of Flemingsberg Campus (SICOF) that receives funding from the Infrastructure Board at Karolinska Institutet.Mice were kept on a 12h light/dark cycle and food and water were available ad libitum. Mice were deeply anesthetized using isoflurane to collect spleen. Dissected spleens were processed to collect splenic immune cells. After isolation, the splenocytes were counted and the viability was measured with the Luna FL dual fluorescence cell counter with dual stain Acridine Orange/Propidium Iodide, with cell size gated between 1-60 μm (Logos Biosystems). Cell suspensions with viability of 85% were encapsulated using the Next Gem Single Cell 3′ Reagent kit v3.1. (Dual index) from 10x Genomics (cat: PN-100026), aiming to capture 5,000 cells. Libraries were prepared as per manufacturer’s instruction (protocol CG000315) and sequenced using the Illumina platform on a Next seq2000 P3 100 cycles flow cell, aiming for an average read depth of 40,000 reads/cell. The cell capture and library generation were performed at the Single Cell Core Facility of Flemingsberg Campus (SICOF) that receives funding from the Infrastructure Board at Karolinska Institutet. Laboratory experimentLaboratory experiment LaboratorieexperimentLaboratorieexperiment Access to data through SND. Data are freely accessible. Åtkomst till data via SND. Data är fritt tillgängliga. openAccess