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        <r:String xml:lang="sv">Data för: Persistent and Compartmentalized T cell Immunity in ALS</r:String>
        <r:String xml:lang="en">Data for: Persistent and Compartmentalized T cell Immunity in ALS</r:String>
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          <r:String xml:lang="sv">Karolinska Institutet</r:String>
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          <r:String xml:lang="sv">Karolinska Institutet</r:String>
          <r:String xml:lang="en">Karolinska Institutet</r:String>
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        <r:SimpleDate>2026-10-12</r:SimpleDate>
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      <r:Content xml:lang="sv">Detta projekt syftade till att förstå aktiveringskraven och den tidsmässiga dynamiken hos T-celler i de perifera och intratekala kompartmenten vid amyotrofisk lateralskleros (ALS). Föreliggande data avser encellssekvensering isolerad från humana cerebrospinalvätska (CSF)-celler och blodprover från nydiagnostiserade ALS-patienter (n=17) och ALS-uppföljning (n=4). Denna analys genomfördes som en del av ett större projekt som sammanfattas i avsnittet nedan.

Med hjälp av encells-RNA och TCR-sekvensering i kombination med avbildningsflödescytometri av celler härledda från cerebrospinalvätska (CSF) och blod identifierade vi en ökad mängd antigenpresenterande cell:T-cellskomplex i CSF, vilket tyder på lokal aktivering. Klonal T-cellsexpansion i CSF observerades med hög interpatientvariabilitet, vilket indikerar immunologisk heterogenitet bland ALS-patienter. Det är värt att notera att CMV-specifika T-celler i CSF, men inte blod, korrelerade positivt med sjukdomsprogressionshastigheten, vilket implicerar en kronisk viral komponent i ALS. Longitudinell flödescytometrisk analys visade förändringar i T-cellsammansättningen efter ALS-diagnos, med perifer T-cellsexpansion kopplad till snabbare progression, medan ökade CSF T-cellsnivåer korrelerade med bättre prognos. Dessa fynd ger viktiga insikter i ALS-immunopatologi och lägger grunden för att utveckla riktade immunterapier.

Datasetet består av 248 GNU-zippade FASTQ-filer. Datasetets totala storlek uppgår till ca 1,1 TB</r:Content>
      <r:Content xml:lang="en">In this project, we aimed to understand the activation requirements and temporal dynamics of T cells in the peripheral and intrathecal compartments in amyotrophic lateral sclerosis (ALS). The present data is on single-cell sequencing isolated from human cerebrospinal fluid (CSF) cells and blood samples from newly diagnosis ALS patients (n=17) and ALS follow up (n=4). This analysis was conducted as part of a bigger project which is summarized in the section below.

Using single-cell RNA and TCR sequencing in combination with imaging flow cytometry of cells derived from cerebrospinal fluid (CSF) and blood, we identified increased amount of antigen-presenting cell:T cell complexes in CSF, suggesting local activation. Clonal T cell expansion in CSF was observed with high inter-patient variability, indicating immunological heterogeneity among ALS patients. Notably, CMV-specific T cells in CSF, but not blood, positively correlated with the rate of disease progression, implicating a chronic viral component in ALS. Longitudinal flow cytometric analysis revealed shifts in T cell composition after ALS diagnosis, with peripheral T cell expansion linked to faster progression, whereas increased CSF T cell levels correlated with better prognosis. These findings provide critical insights into ALS immunopathology and lay the groundwork for developing targeted immunotherapies.

The dataset consists of 248 GNU zipped FASTQ files. The total size of the dataset is approximately 1.1 TB.</r:Content>
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        <r:Keyword xml:lang="sv" controlledVocabularyID="D019636" controlledVocabularyName="MeSH">Neurodegenerativa sjukdomar</r:Keyword>
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