Data för: Single-cell sequencing reveals transcriptional and immunogenetic diversity in stereotyped chronic lymphocytic leukemia Data for: Single-cell sequencing reveals transcriptional and immunogenetic diversity in stereotyped chronic lymphocytic leukemia 2026-29-1 https://doi.org/10.48723/ep36-6r74 Swedish National Data Service Svensk nationell datatjänst Landing page Data för: Single-cell sequencing reveals transcriptional and immunogenetic diversity in stereotyped chronic lymphocytic leukemia Data for: Single-cell sequencing reveals transcriptional and immunogenetic diversity in stereotyped chronic lymphocytic leukemia 2026-29-1 https://doi.org/10.48723/ep36-6r74 oai:swepub.ki.se:1141660 10.1016/j.bneo.2026.100282 Rosenquist, Richard Rosenquist, Richard 22 2448 Pj 22 2448 Pj 2024-02701 2024-02701 ALF/FoUI-962423 ALF/FoUI-962423 194133 194133 Swedish National Data Service Svensk nationell datatjänst Landing page Genetics, Medical Medicinsk genetik Immunogenetics Immungenetik Immunoglobulins Immunglobuliner Receptors, Antigen, B-Cell Receptorer, antigen, B-celler Leukemia, Lymphocytic, Chronic, B-Cell Kronisk B-cellsleukemi Computational Biology Beräkningsbiologi Single-Cell Analysis Singelcellanalys RNA-Seq RNA-seq Single-Cell Gene Expression Analysis Analys av genuttryck i enskilda celler Specific diseases, disorders and medical conditions Specifika sjukdomar, störningar och medicinska tillstånd The dataset contains raw single-cell sequencing data generated from a study of 13 individuals with chronic lymphocytic leukemia (CLL). The patients belong to three biologically defined patient subgroups in which leukemic B cells express highly similar (often near-identical) B-cell receptor immunoglobulin sequences, namely stereotyped subsets #1, #2, and #4. The data were generated using single-cell RNA sequencing (10x Genomics Single Cell 5′ Gene Expression and V(D)J) and include information on gene expression, B-cell receptor repertoires, and T-cell receptor repertoires. The dataset consists of raw sequencing files (FASTQ format) and associated technical metadata. The dataset consists of 138 FASTQ files with a total size of approximately 511 GB. Datasetet innehåller rådata från enkelcellssekvensering genererade i en studie av 13 individer med kronisk lymfatisk leukemi (KLL). Patienterna tillhör tre biologiskt definierade patientsubgrupper där leukemiska B-celler uttrycker mycket likartade (ofta nära identiska) B-cellsreceptor immunoglobulinsekvenser, nämligen stereotypa subset #1, #2 och #4. Data har genererats med enkelcells-RNA-sekvensering (10x Genomics Single Cell 5′ Gene Expression and V(D)J) och omfattar information om genuttryck, B-cellsreceptorrepertoarer samt T-cellsreceptorrepertoarer. Datasetet består av råa sekvenseringsfiler (FASTQ-format) och tillhörande teknisk metadata. Datasetet består av 138 FASTQ-filer. Datasetets totala storlek är ca 511 GB. 2019 2023 IndividualIndividual IndividIndivid CellsCells CellerCeller 13 individuals diagnosed with chronic lymphocytic leukemia, belonging to stereotyped subsets #1, #2, or #4. 13 individer diagnostiserade med kronisk lymfatisk leukemi, tillhörande stereotypa subgrupper #1, #2 eller #4. Numeric Text Other Cross-sectionCross-section TvärsnittTvärsnitt Samples were obtained from individuals diagnosed with chronic lymphocytic leukemia (CLL) belonging to stereotyped subsets #1, #2, and #4. These subsets represent biologically defined patient subgroups in which leukemic B cells express highly similar (often near-identical) B-cell receptor immunoglobulin sequences and constitute only a small proportion of CLL cases (~5%) Due to the limited prevalence of these subsets, individuals were selected based on sample availability. Additional selection criteria included treatment-naïve status at the time of sampling and sufficient sample quality for single-cell analysis, including high cellular viability. Single-cell suspensions were prepared from patient-derived peripheral blood mononuclear cells (PBMCs) and processed for single-cell sequencing.Samples were obtained from individuals diagnosed with chronic lymphocytic leukemia (CLL) belonging to stereotyped subsets #1, #2, and #4. These subsets represent biologically defined patient subgroups in which leukemic B cells express highly similar (often near-identical) B-cell receptor immunoglobulin sequences and constitute only a small proportion of CLL cases (~5%) Due to the limited prevalence of these subsets, individuals were selected based on sample availability. Additional selection criteria included treatment-naïve status at the time of sampling and sufficient sample quality for single-cell analysis, including high cellular viability. Single-cell suspensions were prepared from patient-derived peripheral blood mononuclear cells (PBMCs) and processed for single-cell sequencing. Prover erhölls från individer diagnostiserade med kronisk lymfatisk leukemi (KLL) som tillhörde stereotypa subset #1, #2 och #4. Dessa subset representerar biologiskt definierade patientsubgrupper där leukemiska B-celler uttrycker mycket likartade (ofta nära identiska) B-cellsreceptor immunoglobulinsekvenser och utgör endast en liten andel av KLL-fallen (~5 %). Med hänsyn till den begränsade förekomsten av dessa subset valdes individer baserat på provtillgänglighet. Ytterligare urvalskriterier inkluderade behandlingsnaiv status vid provtagning samt tillräcklig provkvalitet för enkelcellsanalys, inklusive hög cellulär viabilitet. Enkelcellssuspensioner framställdes från patienthärledda perifera blodmononukleära celler (PBMC) och bearbetades för enkelcellssekvensering.Prover erhölls från individer diagnostiserade med kronisk lymfatisk leukemi (KLL) som tillhörde stereotypa subset #1, #2 och #4. Dessa subset representerar biologiskt definierade patientsubgrupper där leukemiska B-celler uttrycker mycket likartade (ofta nära identiska) B-cellsreceptor immunoglobulinsekvenser och utgör endast en liten andel av KLL-fallen (~5 %). Med hänsyn till den begränsade förekomsten av dessa subset valdes individer baserat på provtillgänglighet. Ytterligare urvalskriterier inkluderade behandlingsnaiv status vid provtagning samt tillräcklig provkvalitet för enkelcellsanalys, inklusive hög cellulär viabilitet. Enkelcellssuspensioner framställdes från patienthärledda perifera blodmononukleära celler (PBMC) och bearbetades för enkelcellssekvensering. Non-probability: AvailabilityNon-probability: Availability Icke-sannolikhetsurval: tillgänglighetsurvalIcke-sannolikhetsurval: tillgänglighetsurval Non-probability: PurposiveNon-probability: Purposive Icke-sannolikhetsurval: syftesurvalIcke-sannolikhetsurval: syftesurval Enkelcells-RNA-sekvensering (10x Genomics Single Cell 5′ Gene Expression and V(D)J)Enkelcells-RNA-sekvensering (10x Genomics Single Cell 5′ Gene Expression and V(D)J) Single-cell RNA sequencing (10x Genomics Single Cell 5′ Gene Expression and V(D)J)Single-cell RNA sequencing (10x Genomics Single Cell 5′ Gene Expression and V(D)J) ExperimentExperiment ExperimentExperiment Access to data through SND. Access to data is restricted. Åtkomst till data via SND. Tillgång till data är begränsad. restrictedAccess Oder, B., Rabbani, L., del, P. S. T., Parikh, S., Plevova, K., Mansouri, L., Chatzidimitriou, A., Pospisilova, S., Kay, N., & Stamatopoulos, K. (2026). Single-cell sequencing reveals transcriptional and immunogenetic diversity in stereotyped chronic lymphocytic leukemia. In BLOOD NEOPLASIA (pp. 100282–100282). https://doi.org/10.1016/j.bneo.2026.100282 Oder, B., Rabbani, L., del, P. S. T., Parikh, S., Plevova, K., Mansouri, L., Chatzidimitriou, A., Pospisilova, S., Kay, N., & Stamatopoulos, K. (2026). Single-cell sequencing reveals transcriptional and immunogenetic diversity in stereotyped chronic lymphocytic leukemia. In BLOOD NEOPLASIA (pp. 100282–100282). https://doi.org/10.1016/j.bneo.2026.100282 10.1016/j.bneo.2026.100282 oai:swepub.ki.se:1141660 2026