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            <a:FirstGiven>Mingzhi</a:FirstGiven>
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              <r:String>Margareta Wilhelm</r:String>
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      <r:Title>
        <r:String xml:lang="sv">Data för: ALK R1275Q-mutationen driver expansion av SCP-liknande celler under sympatoadrenal engagemang och initierar neuroblastom</r:String>
        <r:String xml:lang="en">Data for: ALK R1275Q mutation drives expansion of SCP-like cells during sympathoadrenal commitment and primes neuroblastoma initiation</r:String>
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          <r:String xml:lang="sv">Karolinska Institutet</r:String>
          <r:String xml:lang="en">Karolinska Institutet</r:String>
        </r:PublisherName>
      </r:Publisher>
      <r:Publisher>
        <r:PublisherName>
          <r:String xml:lang="sv">Karolinska Institutet</r:String>
          <r:String xml:lang="en">Karolinska Institutet</r:String>
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      <r:PublicationDate>
        <r:SimpleDate>2026-09-08</r:SimpleDate>
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        <r:IdentifierContent>10.48723/9bm1-gr40</r:IdentifierContent>
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      <r:Content xml:lang="sv">Översikt Denna datauppsättning innehåller råa och bearbetade transkriptomiska profileringsdata genererade från en modell av sympatoadrenal (SA) härstamningsdifferentiering baserad på människainducerade pluripotenta stamceller (iPSC). Studien undersöker rollen av ALK R1275Q-mutationen i neuroblastominitiering. Datauppsättningen inkluderar encellig RNA-sekvensering (scRNA-seq) och bulk-RNA-sekvenseringsdata härledda från friska kontroll-iPSC:er och patienthärledda iPSC:er som bär germline-mutationen ALK R1275Q.

Datagenerering och metoder Humana iPSC:er (kontrolllinjer: Ctrl7, Ctrl10, Ctrl14; patientlinjer: NB1, NB2, NB4) differentierades till stamceller i neural crest (tNCC), sympatoadrenala progenitorceller (SAP) och mogna sympatoadrenala celler (SAM).

Encellig RNA-sekvensering (scRNA-seq):

Plattform: 10x Genomics Single Cell 3' v3.1.

Sekvensering: Utförd på MGI DNBSEQ PE100.

Prover: Insamlade vid fyra utvecklingsstadier: tNCC, SAP, dag 8 (D8) och dag 12 (D12) från Ctrl10, NB1, NB2 och NB4.

Bulk-RNA-sekvensering:

Plattform: Illumina PE150-sekvensering.

Prover: Insamlade vid sex utvecklingsstadier: tNCC, SAP, dag 6, dag 8, dag 10 och dag 12 från Ctrl7, Ctrl10, Ctrl14, NB1, NB2 och NB4

Filinnehåll och organisation Datasetet består av följande filtyper:

Råsekvenseringsdata (FASTQ):

Obearbetade sekvenseringsläsningar i .fastq.gz-format.

För scRNA-seq: Parade ändar (t.ex. R1 som innehåller cellstreckkoder/UMI, R2 som innehåller transkriptinsatsen).

För Bulk RNA-sekvensering: Parade ändar (R1/R2).

Filnamn anger vanligtvis prov-ID, bibliotek (GE eller HTO), filnummer och läsriktning (t.ex. SampleID_GE/HTO_L001_R1_001.fastq.gz).

Bearbetade data:

Genuttrycksmatriser: Räknematriser (t.ex. .mtx, .tsv) genererade av Cell Ranger (för scRNA-sekvensering) eller Salmon (för bulk RNA-sekvensering).

Metadata: Tabeller som innehåller provinformation och differentieringstidpunkter.</r:Content>
      <r:Content xml:lang="en">Overview This dataset contains raw and processed transcriptomic profiling data generated from a human induced pluripotent stem cell (iPSC)-based model of sympathoadrenal (SA) lineage differentiation. The study investigates the role of the ALK R1275Q mutation in Neuroblastoma initiation. The dataset includes single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing data derived from healthy control iPSCs and patient-derived iPSCs carrying the germline ALK R1275Q mutation.

The dataset consists of approximately 500 files totalling approximately 486 GB

Data Generation and Methods Human iPSCs (Control lines: Ctrl7, Ctrl10, Ctrl14; Patient lines: NB1, NB2, NB4) were differentiated into trunk neural crest cells (tNCCs), sympathoadrenal progenitors (SAPs), and mature sympathoadrenal cells (SAMs).

Single-Cell RNA Sequencing (scRNA-seq):

Platform: 10x Genomics Single Cell 3' v3.1.

Sequencing: Performed on MGI DNBSEQ PE100.

Samples: Collected at four developmental stages: tNCC, SAP, Day 8 (D8), and Day 12 (D12) from Ctrl10, NB1, NB2, and NB4.

Bulk RNA Sequencing:

Platform: Illumina PE150 sequencing.

Samples: Collected at six developmental stages: tNCC, SAP, Day 6, Day 8, Day 10 and Day 12 from Ctrl7, Ctrl10, Ctrl14, NB1, NB2, and NB4

File Content and Organization The dataset consists of the following file types:

Raw Sequencing Data (FASTQ):

Unprocessed sequencing reads in .fastq.gz format.

For scRNA-seq: Paired-end reads (e.g., R1 containing cell barcodes/UMIs, R2 containing the transcript insert).

For Bulk RNA-seq: Paired-end reads (R1/R2).

Filenames typically indicate sample ID, library (GE or HTO), lane number, and read direction (e.g., SampleID_GE/HTO_L001_R1_001.fastq.gz).

Processed Data:

Gene Expression Matrices: Count matrices (e.g., .mtx, .tsv) generated by Cell Ranger (for scRNA-seq) or Salmon (for bulk RNA-seq).

Metadata: Tables containing sample information and differentiation time points.</r:Content>
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        <r:Subject xml:lang="en" controlledVocabularyID="30108" controlledVocabularyName="Standard för svensk indelning av forskningsämnen 2025">Cell and Molecular Biology</r:Subject>
        <r:Subject xml:lang="sv" controlledVocabularyID="30108" controlledVocabularyName="Standard för svensk indelning av forskningsämnen 2025">Cell- och molekylärbiologi</r:Subject>
        <r:Subject xml:lang="en" controlledVocabularyID="30112" controlledVocabularyName="Standard för svensk indelning av forskningsämnen 2025">Basic Cancer Research</r:Subject>
        <r:Subject xml:lang="sv" controlledVocabularyID="30112" controlledVocabularyName="Standard för svensk indelning av forskningsämnen 2025">Basal cancerforskning</r:Subject>
        <r:Keyword xml:lang="en" controlledVocabularyID="D009432" controlledVocabularyName="MeSH">Neural Crest</r:Keyword>
        <r:Keyword xml:lang="sv" controlledVocabularyID="D009432" controlledVocabularyName="MeSH">Neurallist</r:Keyword>
        <r:Keyword xml:lang="en" controlledVocabularyID="D009447" controlledVocabularyName="MeSH">Neuroblastoma</r:Keyword>
        <r:Keyword xml:lang="sv" controlledVocabularyID="D009447" controlledVocabularyName="MeSH">Neuroblastom</r:Keyword>
        <r:Keyword xml:lang="en" controlledVocabularyID="D057026" controlledVocabularyName="MeSH">Induced Pluripotent Stem Cells</r:Keyword>
        <r:Keyword xml:lang="sv" controlledVocabularyID="D057026" controlledVocabularyName="MeSH">Inducerade pluripotenta stamceller</r:Keyword>
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