Thermal Proteome Profiling dataset from Hazara virus infected SW13 cells treated with DMSO or antiviral inhibitor 20 µM TH6744.
type: Dataset
description: Article: S3 Dataset from a publication "Tampere, M.; Pettke, A.; Salata, C.; Wallner, O.; Koolmeister, T.; Cazares-Körner, A.; Visnes, T.; Hesselman, M.C.; Kunold, E.; Wiita, E.; Kalderén, C.; Lightowler, M.; Jemth, A.-S.; Lehtiö, J.; Rosenquist, Å.; Warpman-Berglund, U.; Helleday, T.; Mirazimi, A.; Jafari, R.; Puumalainen, M.-R. Novel Broad-Spectrum Antiviral Inhibitors Targeting Host Factors Essential for Replication of Pathogenic RNA Viruses. Viruses 2020, 12, 1423." Abstract: Recent RNA virus outbreaks such as Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Ebola virus (EBOV) () have caused worldwide health emergencies highlighting the urgent need for new antiviral strategies. Targeting host cell pathways supporting viral replication is an attractive approach for development of antiviral compounds, especially with new, unexplored viruses where knowledge of virus biology is limited. Here, we present a strategy to identify host-targeted small molecule inhibitors using an image-based phenotypic antiviral screening assay followed by extensive target identification efforts revealing altered cellular pathways upon antiviral compound treatment. The newly discovered antiviral compounds showed broad-range antiviral activity against pathogenic RNA viruses such as SARS-CoV-2, EBOV and Crimean-Congo hemorrhagic fever virus (CCHFV). Target identification of the antiviral compounds by thermal protein profiling revealed major effects on proteostasis pathways and disturbance in interactions between cellular HSP70 complex and viral proteins, illustrating the supportive role of HSP70 on many RNA viruses across virus families. Collectively, this strategy identifies new small molecule inhibitors with broad antiviral activity against pathogenic RNA viruses, but also uncovers novel virus biology urgently needed for design of new antiviral therapies. Data processing: Current processed dataset in Excel format originates from Thermal Proteome Profiling (TPP-TR) performe…
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: Marjo Puumalainen
  type: Person
  family name: Puumalainen
  given name: Marjo
creator: Marianna Tampere
creator: Rozbeh Jafari
date modified: 2023-05-30
date published: 2020-12-11
keywords: Medical and Health Sciences
  type: DefinedTerm
  term code: 3
keywords: Biochemistry
  type: DefinedTerm
  term code: 10408
keywords: Microbiology
  type: DefinedTerm
  term code: 10606
keywords: Microbiology not elsewhere classified
keywords: Virology
keywords: Biochemistry and cell biology not elsewhere classified
license: https://creativecommons.org/licenses/by/4.0/
publisher: Karolinska Institutet
version: 0

PropertyValue
property ID: DOI
value: 10.17044/SCILIFELAB.13089023

Marianna Tampere
type: Person
family name: Tampere
given name: Marianna

Rozbeh Jafari
type: Person
family name: Jafari
given name: Rozbeh

Karolinska Institutet
type: Organization