Single base substitution mutational signatures in pediatric acute myeloid leukemia based on whole genome sequencing doi-10-17044-scilifelab-13325822-0 https://doi.org/10.17044/SCILIFELAB.13325822 Swedish National Data Service Svensk nationell datatjänst Landing page Single base substitution mutational signatures in pediatric acute myeloid leukemia based on whole genome sequencing doi-10-17044-scilifelab-13325822-0 https://doi.org/10.17044/SCILIFELAB.13325822 Gunnarsson, Rebeqa Yang, Minjun 2020-01164_VR Swedish National Data Service Svensk nationell datatjänst Landing page This dataset includes whole genome sequencing (WGS) data of 20 diagnostic, and 20 remission samples from 20 children/adolescents with acute myeloid leukemia (AML), treated at the Departments of Pediatrics at Lund and Linköping University Hospitals between 1994 and 2016. The median age of the patients was 8 years (range 0-17 years) and the female/male ratio was 1:1. DNA was extracted from diagnostic bone marrow (BM; n = 17)/peripheral blood (PB; n = 3) samples, remission BM (n = 15)/PB (n = 5), and from two BM relapses. Construction of libraries, using the TruSeq Nano DNA sample preparation kit (Illumina, San Diego, CA, USA) on 100 ng DNA, and massively parallel sequencing were performed by BGI Tech Solutions (Hong Kong). The WGS (Illumina HiSeqX) reached an average sequencing depth of 30-42x/sample (median 33x), with 2x 150 bp read length. Access to data through an external actor. Access to data is restricted. Åtkomst till data via extern aktör. Tillgång till data är begränsad. restrictedAccess