Archaic humans have contributed to large-scale variation in modern human T cell receptor genes
type: Dataset
description: The dataset contains the repertoire sequencing data from 45 adult volunteers. For each case 4 separate TCR repseq libraries were constructed after cDNA synthesis using a UMI containing primer located in the constant exon of the TRA, TRD, TRB and TRG genes respectively. In each case a 5’ multiplex set of primers specific for the leader regions of the V genes of either TRA, TRD, TRB or TRG genes was used along with a universal reverse primer, introduced during the cDNA synthesis step to produce the gene specific repseq library. The libraries were sequenced using the Illumina 2 x 300 cycle MiSeq system at the Karlsson Hedestam Laboratory at the Department of Microbiology, Tumor and Cell Biology at Biomedicum, Karolinska Institutet, Stockholm. Terms for access The datasets are only to be used for research that is seeking to advance the understanding of the influence of TCR genetic variation on human biology.
conditions of access: https://publications.europa.eu/resource/authority/access-right/NON_PUBLIC
conditions of access: info:eu-repo/semantics/restrictedAccess
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: Martin Corcoran
  type: Person
  family name: Corcoran
  given name: Martin
creator: Mark Chernyshev
  type: Person
  family name: Chernyshev
  given name: Mark
creator: Marco Mandolesi
  type: Person
  family name: Mandolesi
  given name: Marco
creator: Sanjana Narang
  type: Person
  family name: Narang
  given name: Sanjana
creator: Mateusz Kaduk
  type: Person
  affiliation: Science for Life Laboratory
  family name: Kaduk
  given name: Mateusz
creator: Christopher Sundling
  type: Person
  family name: Sundling
  given name: Christopher
creator: Anna Färnert
  type: Person
  family name: Färnert
  given name: Anna
creator: Carolina Bernhardsson
  type: Person
  family name: Bernhardsson
  given name: Carolina
creator: Maximilian Larena
  type: Person
  family name: Larena
  given name: Maximilian
creator: Mattias Jakobsson
  type: Person
  family name: Jakobsson
  given name: Mattias
creator: Gunilla B. Karlsson Hedestam
  type: Person
  family name: B. Karlsson Hedestam
  given name: Gunilla
date modified: 2024-12-13
date published: 2023-03-17
keywords: Medical and Health Sciences
  type: DefinedTerm
  term code: 3
keywords: Immunology
  type: DefinedTerm
  term code: 10605
keywords: Genetic immunology
publisher: Karolinska Institutet
version: 0

PropertyValue
property ID: DOI
value: 10.17044/SCILIFELAB.14579091

Science for Life Laboratory
type: Organization

Karolinska Institutet
type: Organization