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            <a:FirstGiven>Mattias</a:FirstGiven>
            <a:LastFamily>Pilheden</a:LastFamily>
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              <r:String>Mattias Pilheden</r:String>
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        <r:URN>urn:ddi:se.researchdata:doi-10-17044-scilifelab-19380116.Individual-0:2.0</r:URN>
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            <a:FirstGiven>Anna</a:FirstGiven>
            <a:LastFamily>Hagström Andersson</a:LastFamily>
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              <r:String>Anna Hagström Andersson</r:String>
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      <r:Title>
        <r:String xml:lang="en">Duplex sequencing uncovers recurrent low-frequency cancer-associated mutations in KMT2A-rearranged infant acute leukemia</r:String>
      </r:Title>
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          <r:URN>urn:ddi:se.researchdata:doi-10-17044-scilifelab-19380116.Individual-0:2.0</r:URN>
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          <r:String xml:lang="sv">Lunds universitet</r:String>
          <r:String xml:lang="en">Lund University</r:String>
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      <r:Publisher>
        <r:PublisherName>
          <r:String xml:lang="sv">Lunds universitet</r:String>
          <r:String xml:lang="en">Lund University</r:String>
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      <r:PublicationDate>
        <r:SimpleDate>2022-04-21</r:SimpleDate>
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        <r:IdentifierContent>10.17044/SCILIFELAB.19380116</r:IdentifierContent>
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      <r:Content xml:lang="en">This dataset includes Duplex sequencing and targeted next generation sequencing (NGS) of 20 Infants and 7 Children with acute lymphoblastic leukmia.

All patients had an underlying rearrangement of the KMT2A gene, either KMT2A::MLLT3 (n=5), KMT2A::AFF1 (n=12), KMT2A::MLLT1 (n=8) or KMT2A::MLLT10 (n=2). The median age of the cohort was 7 months. 

Diagnostic sample from peripheral blod (n=11) or bone marrow (n=16) underwent the duplex sequencing library preparation as described in https://doi.org/10.1073/pnas.1208715109 and sequenced on the NextSeq 500 (Illumina, San Diego, CA, USA).

A subset of variants were validated by targeted multiplex-PCR followed by NGS. In six patients with available relapse samples, a screening of diagnostic variants was performed by multiplex-PCR and NGS. All targeted sequencing libraries were prepared with Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced on the Illumina MiSeq.</r:Content>
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