Single-cell gene expression count data for trifluridine treated co-culture of tumor cell line with PBMC
type: Dataset
description: Gene expression (counts) scRNA-seq of co-cultured cancer- and immune cells treated with trifluridine and DMSO control assayed at two time-points (12h and 72h). HCT116 were seeded in 6-well Nunc plates (50,000 cells/3mL/well) and precultured for 24 h before PBMCs were added at a 1:8 ratio. Co-cultures were treated with DMSO vehicle (0.1%) or FTD (3mM) for 12 h or 72 h. MACS Dead Cell Removal Kit (Miltenyi Biotec, Gladbach, DEU) was performed according to the manufacturer’s instructions on cells treated for 72 h to increase the viability of the samples before RNA-sequencing. The viability of the samples treated for 12 h was not subjected to Dead Cell Removal as the viability was already sufficient. All samples were washed in PBS with 0.04% BSA (2x1mL). Chromium Next GEM Single Cell 3’ library preparation and RNA-sequencing were performed by the SNP&SEQ Technology Platform (National Genomics Infrastructure (NGI), Science for Life Laboratory, Uppsala University, Sweden). This data set contains processed data using Cell Ranger toolkit version 5.0.1 provided by 10x Genomics, for demultiplexing, aligning reads to the human reference genome GRCh38, and generating gene-cell unique molecular identifiers
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: Claes Andersson
  type: Person
  affiliation: Science for Life Laboratory
  family name: Andersson
  given name: Claes
creator: Tove Selvin
  type: Person
  family name: Selvin
  given name: Tove
date modified: 2023-05-30
date published: 2022-06-21
keywords: Medical and Health Sciences
  type: DefinedTerm
  term code: 3
keywords: Cancer and Oncology
  type: DefinedTerm
  term code: 30203
keywords: Other Basic Medicine
  type: DefinedTerm
  term code: 30199
keywords: Pharmacology and pharmaceutical sciences not elsewhere classified
keywords: Chemotherapy
keywords: Tumour immunology
license: https://creativecommons.org/licenses/by/4.0/
publisher: Uppsala University
version: 0

PropertyValue
property ID: DOI
value: 10.17044/SCILIFELAB.19761307

Science for Life Laboratory
type: Organization

Uppsala University
type: Organization