Data and code related to "Spatiotemporal kinetics of the SRP pathway in live E. coli cells", by Volkov et al.
type: Dataset
description: This item contains fluorescence microscopy data, simulated data, DNA sequence data, and analysis code related to the publication: Volkov et al., "Spatiotemporal kinetics of the SRP pathway in live E. coli cells". The Signal recognition Particle (SRP) pathway is a universal mechanism for co-translational targeting of nascent polypeptides to the membrane bound translocation complexes, the translocons. By performing single-molecule tracking of dye-labelled SRP in living E. coli cells, we have been able to directly observe and measure the kinetics of this process. We further validated and refined our resulting reaction-diffusion model through simulated microscopy. All raw microscopy data, simulated data, and processed data are included in the item, along with analysis code and code for figure generation. Data has been acquired at the Dept. of Cell and Molecular Biology, Biomedical Center (BMC), Uppsala University. For technical details about data acquisition, please see the related publication Volkov et al. "Spatiotemporal kinetics of the SRP pathway in live E. coli cells". A detailed list of files and file organization can be found in the readme text file.
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: Ivan Volkov
  type: Person
  family name: Volkov
  given name: Ivan
creator: Erik Lundin
  type: Person
  family name: Lundin
  given name: Erik
creator: Kalle Kipper
  type: Person
  family name: Kipper
  given name: Kalle
creator: Mikhail Metelev
  type: Person
  family name: Metelev
  given name: Mikhail
creator: Spartak Zikrin
  type: Person
  family name: Zikrin
  given name: Spartak
creator: Magnus Johansson
date modified: 2023-06-01
date published: 2022-09-08
funding: Single-molecule tracking for live-cell protein synthesis kinetics
  type: Grant
  funder: European Research Council
funding: Studies of protein synthesis in live cells - one molecule at a time
  type: Grant
  funder: Swedish Research Council
funding: The physics of genetic information processing
  type: Grant
  funder: Swedish Research Council
funding: An Integrated Environment for Ribosome Research
  type: Grant
  funder: Swedish Research Council
funding: Determinants for efficient synthesis, folding, and targeting of proteins in living cells
  type: Grant
  funder: Swedish Research Council
keywords: Medical and Health Sciences
  type: DefinedTerm
  term code: 3
keywords: Biochemistry
  type: DefinedTerm
  term code: 10408
keywords: Biochemistry and cell biology not elsewhere classified
license: https://creativecommons.org/licenses/by/4.0/
publisher: Uppsala University
version: 0

PropertyValue
property ID: DOI
value: 10.17044/SCILIFELAB.20502126

Magnus Johansson
type: Person
affiliation: Science for Life Laboratory
family name: Johansson
given name: Magnus

Science for Life Laboratory
type: Organization

European Research Council
type: Organization

Swedish Research Council
type: Organization

Uppsala University
type: Organization