Data and code related to "Spatiotemporal kinetics of the SRP pathway in live E. coli cells", by Volkov et al.
This item contains fluorescence microscopy data, simulated data, DNA sequence data, and analysis code related to the publication: Volkov et al., "Spatiotemporal kinetics of the SRP pathway in live E. coli cells". The Signal recognition Particle (SRP) pathway is a universal mechanism for co-translational targeting of nascent polypeptides to the membrane bound translocation complexes, the translocons. By performing single-molecule tracking of dye-labelled SRP in living E. coli cells, we have been able to directly observe and measure the kinetics of this process. We further validated and refined our resulting reaction-diffusion model through simulated microscopy. All raw microscopy data, simulated data, and processed data are included in the item, along with analysis code and code for figure generation. Data has been acquired at the Dept. of Cell and Molecular Biology, Biomedical Center (BMC), Uppsala University. For technical details about data acquisition, please see the related publication Volkov et al. "Spatiotemporal kinetics of the SRP pathway in live E. coli cells". A detailed list of files and file organization can be found in the readme text file.
Single-molecule tracking for live-cell protein synthesis kinetics
Studies of protein synthesis in live cells - one molecule at a time
The physics of genetic information processing
An Integrated Environment for Ribosome Research
Determinants for efficient synthesis, folding, and targeting of proteins in living cells
Biochemistry and cell biology not elsewhere classified