Atlas of 5'P mRNA decay in bacteria doi-10-17044-scilifelab-22284709-0 https://doi.org/10.17044/SCILIFELAB.22284709 Swedish National Data Service Svensk nationell datatjänst Landing page Atlas of 5'P mRNA decay in bacteria doi-10-17044-scilifelab-22284709-0 https://doi.org/10.17044/SCILIFELAB.22284709 Pelechano, Vicent Swedish National Data Service Svensk nationell datatjänst Landing page Regulation of mRNA stability is a central process in gene expression. Here, report an atlas of 5´ monophosphorylated mRNA decay intermediates (5´P) in 96 species. We use those data to demonstrate that in species with 5´-3´exonuclease activity, the exoribonuclease RNase J follows the trailing ribosome, producing an in vivo single-nucleotide toeprint of its 5´ position. While in other species, ribosome position alters endonucleolytic cleavage sites. Our work demonstrates the universal connection between RNA decay and translation and paves the way for use of metadegradome sequencing to investigate post-transcriptional regulation of unculturable species present and complex microbial communities. Data was analyzed using our fivepseq software.  Improved computational analysis of ribosome dynamics from 5′P degradome data using fivepseq (https://doi.org/10.1093/nargab/lqaa099) . Lilit Nersisyan, Maria Ropat & Vicent Pelechano. NAR Genomics and Bioinformatics, Volume 2, Issue 4, December 2020, lqaa099, https://doi.org/10.1093/nargab/lqaa099 Access to data through an external actor. Åtkomst till data via extern aktör.