This collection contains an example MINUTE-ChIP dataset to run minute pipeline on, provided as supporting material to help users understand the results of a MINUTE-ChIP experiment from raw data to a primary analysis that yields the relevant files for downstream analysis along with summarized QC indicators. Example primary non-demultiplexed FASTQ files provided here were used to generate GSM5493452-GSM5493463 (H3K27m3) and GSM5823907-GSM5823918 (Input), deposited on GEO with the minute pipeline all together under series GSE181241. For more information about MINUTE-ChIP, you can check the publication (https://www.nature.com/articles/s41556-022-00916-w) relevant to this dataset: Kumar, Banushree, et al. "Polycomb repressive complex 2 shields naïve human pluripotent cells from trophectoderm differentiation." Nature Cell Biology 24.6 (2022): 845-857. If you want more information about the minute pipeline, there is a public biorXiv (https://www.biorxiv.org/content/10.1101/2022.03.14.484318v1) and a GitHub repository (https://github.com/elsasserlab/minute) and official documentation (https://minute.readthedocs.io/en/latest/) .
Dynamic interplay of Polycomb complexes in establishing and maintaining cellular memory