Processed ASV data from the Insect Biome Atlas Project
type: Dataset
description: NOTE: The gzipped files in this upload have mistakenly been compressed twice. To decompress the files, please run e.g.: gunzip -c CO1_asv_counts_SE.tsv.gz | gunzip -c > CO1_asv_counts_SE.tsv The Insect Biome Atlas project was supported by the Knut and Alice Wallenberg Foundation (dnr 2017.0088). The project analyzed the insect faunas of Sweden and Madagascar, and their associated microbiomes, mainly using DNA metabarcoding of Malaise trap samples collected in 2019 (Sweden) or 2019–2020 (Madagascar). Please cite this version of the dataset as: Miraldo A, Iwaszkiewicz-Eggebrecht E, Sundh J, Lokeshwaran M, Granqvist E, Goodsell R, Andersson AF, Lukasik P, Roslin T, Tack A, Ronquist F. 2024. Processed ASV data from the Insect Biome Atlas Project, version 3. doi:10.17044/scilifelab.27202368.v3 or https://doi.org/10.17044/scilifelab.27202368.v3 This dataset contains the results from bioinformatic processing of version 1 of the amplicon sequence variant (ASV) data from the Insect Biome Atlas project (Miraldo et al. 2024), that is, the cytochrome oxidase subunit 1 (CO1) metabarcoding data from Malaise trap samples processed using the FAVIS mild lysis protocol (Iwaszkiewicz et al. 2023). The bioinformatic processing involved: (1) taxonomic assignment of ASVs, (2) chimera removal; (3) clustering into OTUs; (4) noise filtering and (5) cleaning. The clustering step involved resolution of the taxonomic annotation of the cluster and identification of a representative ASV. The noise filtering step involved removal of ASV clusters identified as potentially originating from nuclear mitochondrial DNA (NUMTs) or representing other types of error or noise. The cleaning step involved removal of ASV clusters present in >5% of negative control samples. ASV taxonomic assignments, ASV cluster designations, consensus taxonomies and summed counts of clusters in the sequenced samples are provided in compressed tab-separated files. Sequences of cluster representatives are provided in compressed…
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: Andreia Miraldo
  type: Person
  affiliation: Science for Life Laboratory
  family name: Miraldo
  given name: Andreia
creator: Elzbieta Iwaszkiewicz-Eggebrecht
creator: John Sundh
creator: Lokeshwaran Manoharan
creator: Emma Granqvist
  type: Person
  family name: Granqvist
  given name: Emma
creator: Anders F. Andersson
creator: Piotr Łukasik
creator: Tomas Roslin
creator: Ayco J. M. Tack
creator: Fredrik Ronquist
date modified: 2025-05-07
date published: 2024-10-30
funding: Insect Biome Atlas
  type: Grant
  funder: Knut and Alice Wallenberg Foundation
keywords: Medical and Health Sciences
  type: DefinedTerm
  term code: 3
keywords: Ecology
  type: DefinedTerm
  term code: 10611
keywords: Ecology not elsewhere classified
keywords: Conservation and biodiversity
license: https://creativecommons.org/licenses/by/4.0/
publisher: Swedish Museum of Natural History
version: 0

PropertyValue
property ID: DOI
value: 10.17044/SCILIFELAB.27202368

Science for Life Laboratory
type: Organization

Elzbieta Iwaszkiewicz-Eggebrecht
type: Person
family name: Iwaszkiewicz-Eggebrecht
given name: Elzbieta

John Sundh
type: Person
family name: Sundh
given name: John

Lokeshwaran Manoharan
type: Person
family name: Manoharan
given name: Lokeshwaran

Anders F. Andersson
type: Person
family name: Andersson
given name: Anders F.

Piotr Łukasik
type: Person
family name: Łukasik
given name: Piotr

Tomas Roslin
type: Person
family name: Roslin
given name: Tomas

Ayco J. M. Tack
type: Person
family name: Tack
given name: Ayco J. M.

Fredrik Ronquist
type: Person
affiliation: Science for Life Laboratory
family name: Ronquist
given name: Fredrik

Knut and Alice Wallenberg Foundation
type: Organization

Swedish Museum of Natural History
type: Organization