Live-cell Phase Contrast image dataset using a balanced class benchmark assay
type: Dataset
description: Dataset generated for 10.1101/2025.04.22.649936 (https://doi.org/10.1101/2025.04.22.649936) , using an assay with 6 balanced MoA classes. The dataset contains time-lapse images of A549 and U2OS cells. Paired github repository: https://github.com/edvinforsgren/LCTP The data contains time-lapse images of two cell lines (A549 and U2OS). The cells were cultivated in an benchmark assay developed for a paper where analysis of a single time-point versus analysis of the full time-lapse was evaluated as a way to determine if adding temporal data could enhance the mined features and improve downstream tasks. The experimental set-up consists of three biological replicates (i.e. three plates with unique layout variants) for each cell-line (A549 and U2OS) with 5 technical replicates (i.e. 5 wells) and 15 DMSO control wells each - for a total of 15 replicates and 45 DMSO replicates for each cell-line. The time-lapse acquisition was taken with 2 hours between each acquisition over a duration of 72 hours.
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: David Holmberg
creator: Pär Jonsson
creator: Ola Spjuth
creator: Gillian Lovell
creator: Johan Trygg
creator: Jordi Carreras-Puigvert
creator: Martin Johansson
creator: Jonne Rietdijk
creator: Edvin Forsgren
date modified: 2026-01-21
date published: 2026-01-21
keywords: Medical and Health Sciences
  type: DefinedTerm
  term code: 3
keywords: Image processing
keywords: Biomedical imaging
keywords: Cell physiology
license: https://creativecommons.org/licenses/by-nc-sa/4.0/
publisher: Umeå University
version: 0

PropertyValue
property ID: DOI
value: 10.17044/SCILIFELAB.29315642

David Holmberg
type: Person
affiliation: Science for Life Laboratory
family name: Holmberg
given name: David

Science for Life Laboratory
type: Organization

Pär Jonsson
type: Person
family name: Jonsson
given name: Pär

Ola Spjuth
type: Person
family name: Spjuth
given name: Ola

Gillian Lovell
type: Person
family name: Lovell
given name: Gillian

Johan Trygg
type: Person
family name: Trygg
given name: Johan

Jordi Carreras-Puigvert
type: Person
family name: Carreras-Puigvert
given name: Jordi

Martin Johansson
type: Person
family name: Johansson
given name: Martin

Jonne Rietdijk
type: Person
family name: Rietdijk
given name: Jonne

Edvin Forsgren
type: Person
family name: Forsgren
given name: Edvin

Umeå University
type: Organization