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        <parTitl xml:lang="en">Sequencing data of DNA nanostructures selected for uptake in cells</parTitl>
        <IDNo agency="SND">doi-10-17044-scilifelab-31323787-0</IDNo>
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    <citation>
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        <parTitl xml:lang="en">Sequencing data of DNA nanostructures selected for uptake in cells</parTitl>
        <IDNo agency="SND">doi-10-17044-scilifelab-31323787-0</IDNo>
        <IDNo agency="DOI">https://doi.org/10.17044/SCILIFELAB.31323787</IDNo>
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        <AuthEnty xml:lang="en" affiliation="Science for Life Laboratory">Benson, Erik</AuthEnty>
        <AuthEnty xml:lang="en" affiliation="Science for Life Laboratory">Rajwar, Anjali</AuthEnty>
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        <grantNo xml:lang="en" agency="Swedish Research Council">2022-04147_VR</grantNo>
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      <abstract xml:lang="en" contentType="abstract">Selection of DNA nanostructures by cellular uptake studied by high throughput sequencing. We generated a library of DNA nanostructures folded from single-stranded genomes and sequenced the initial library on Oxford nanopore (ONT) and Illumina. The data from this is stored in the files:

Initial_library_ONT.fastq

Initial_library_Illumina_merged.assembled.fastq





We then performed repeated selection experiment in the cell lines HEK293T and RAW 264.7 combined with sequencing to monitor the process. The data from HEK293T round 4,6,8 and 10 sequenced on Oxford nanopore is stored in the files: 

HEK293_R4_ONT.fastq

HEK293_R6_ONT.fastq

HEK293_R8_ONT.fastq

HEK293_R10_ONT.fastq

The data from RAW264.7 round 4,6,8 and 10 sequenced on Oxford nanopore is stored in the files: 

Raw_R4_ONT.fastq

Raw_R6_ONT.fastq

Raw_R8_ONT.fastq

Raw_R10_ONT.fastq

The data from HEK293T round 5 and 10 sequenced on illumina is stored in the files: 

HEK293_R5_illumina_merged.assembled.fastq

HEK293_R10_illumina_merged.assembled.fastq

The data from RAW264.7 round 5 and 10 sequenced on illumina is stored in the files: 

RAW_R5_Illumina_merged.assembled.fastq

RAW_R10_Illumina_merged.assembled.fastq</abstract>
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