Sequencing data of DNA nanostructures selected for uptake in cells
type: Dataset
description: Selection of DNA nanostructures by cellular uptake studied by high throughput sequencing. We generated a library of DNA nanostructures folded from single-stranded genomes and sequenced the initial library on Oxford nanopore (ONT) and Illumina. The data from this is stored in the files: Initial_library_ONT.fastq Initial_library_Illumina_merged.assembled.fastq We then performed repeated selection experiment in the cell lines HEK293T and RAW 264.7 combined with sequencing to monitor the process. The data from HEK293T round 4,6,8 and 10 sequenced on Oxford nanopore is stored in the files: HEK293_R4_ONT.fastq HEK293_R6_ONT.fastq HEK293_R8_ONT.fastq HEK293_R10_ONT.fastq The data from RAW264.7 round 4,6,8 and 10 sequenced on Oxford nanopore is stored in the files: Raw_R4_ONT.fastq Raw_R6_ONT.fastq Raw_R8_ONT.fastq Raw_R10_ONT.fastq The data from HEK293T round 5 and 10 sequenced on illumina is stored in the files: HEK293_R5_illumina_merged.assembled.fastq HEK293_R10_illumina_merged.assembled.fastq The data from RAW264.7 round 5 and 10 sequenced on illumina is stored in the files: RAW_R5_Illumina_merged.assembled.fastq RAW_R10_Illumina_merged.assembled.fastq
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: Erik Benson
  type: Person
  affiliation: Science for Life Laboratory
  family name: Benson
  given name: Erik
creator: Anjali Rajwar
  type: Person
  affiliation: Science for Life Laboratory
  family name: Rajwar
  given name: Anjali
creator: Sandra Ly
  type: Person
  family name: Ly
  given name: Sandra
creator: Lisa Eichhorn
  type: Person
  family name: Eichhorn
  given name: Lisa
creator: Jakub Palacka
  type: Person
  family name: Palacka
  given name: Jakub
date modified: 2026-03-12
date published: 2026-03-12
funding: Combinatorially generated sequenceable DNA nanostructure libraries for one-pot development of high affinity binders via an evolutionary selection process.
  type: Grant
  funder: Swedish Research Council
keywords: Medical and Health Sciences
  type: DefinedTerm
  term code: 3
keywords: Nanomedicine
license: https://creativecommons.org/licenses/by/4.0/
publisher: Karolinska Institutet
version: 0

PropertyValue
property ID: DOI
value: 10.17044/SCILIFELAB.31323787

Science for Life Laboratory
type: Organization

Swedish Research Council
type: Organization

Karolinska Institutet
type: Organization