Integrative Analysis of Left Ventricle and Epicardial Adipose Tissue Identifies SDHA and OGDH as Candidate Targets for Ischemic Heart Disease
type: Dataset
distribution: Landing page for the dataset
description: Sample Collections from Human SubjectsCardiac ischemic biopsies were obtained from 30 patients undergoing coronary artery bypass surgery at the Sahlgrenska University Hospital, Gothenburg, Sweden, due to significant atherosclerotic blockage in the epicardial coronary arteries. The surgery and sample collections were performed between 7 February 2013 and 1 February 2017. All patients were examined through echocardiography prior to operation, and the ejection fraction (EF) was measured. Non-ischemic biopsies from the left ventricle were obtained from 14 subjects undergoing aortic valve replacement in the same hospital, with angiography verified absence of coronary artery disease in any of the major myocardial coronary artery branches. Individual level data on age, sex, and other information is available in Data S1. All biopsies were collected from the left ventricle septum region with a 1 mm needle. The average weight of collected tissues samples was 100 mg and these were stored at -80°C until analysis. All patients gave informed and written consent. Patient characteristics (including diabetes status) were either collected as the patients entered the study or retrieved later from their medical charts. The diagnoses were based on ICD-codes from patient records and national databases. The study was approved by the Gothenburg Regional Ethics Committee and done according to the Declaration of Helsinki (Dnr 064-14). RNA extraction and sequencingTotal RNA was isolated from biopsies relating to 44 samples from the left ventricle using RNeasy Fibrous Tissue Mini kit (QIAGEN) and 24 samples from epicardial fat using RNeasy Lipid Tissue Mini kit (QIAGEN). These 68 samples were processed with SMARTer® Stranded RNA-Seq Kit (Takara Bio) for reverse transcription, generation of double stranded cDNA and subsequent library preparation. All libraries were quantified with the Fragment Analyzer using the standard sensitivity NGS kit (Agilent Technologies), pooled in equimolar concentrat…
modified: 2026-05-20T00:00:00Z
issued: 2026-05-20T00:00:00Z
publisher: University of Gothenburg
theme: HEAL
access rights: NON PUBLIC
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Muhammad Arif
    type: Agent
    type: PrivateIndividual(s)
keyword: Systems biology

Landing page for the dataset
type: Distribution
title: Landningssida för datasetet @sv
access URL: Integrative Analysis of Left Ventricle and Epicardial Adipose Tissue Identifies SDHA and OGDH as Candidate Targets for Ischemic Heart Disease
availability: stable

University of Gothenburg
type: Agent
name: Göteborgs universitet @sv
type: Academia-ScientificOrganisation
source: 01tm6cn81