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        <r:String xml:lang="en">Rodrigues et al. Fluctuating heat stress data</r:String>
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          <r:String xml:lang="sv">Stockholms universitet</r:String>
          <r:String xml:lang="en">Stockholm University</r:String>
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        <r:PublisherName>
          <r:String xml:lang="sv">Stockholms universitet</r:String>
          <r:String xml:lang="en">Stockholm University</r:String>
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      <r:PublicationDate>
        <r:SimpleDate>2021-11-09</r:SimpleDate>
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      <r:Content xml:lang="en">Summary:


This data was used to assess how fluctuating
sublethal heat stress during development impacted subsequent male reproductive performance
and success. Using a subset of isogenic lines of the Drosophila Genetic
Reference Panel (DGRP) reared at standardized densities in four thermal
conditions (Constant 25°C, Constant 29°C, Fluctuating 25°C, Fluctuating 29°C),
we quantified phenotypic (and genetic) variation in mating propensity, fertility,
productivity and sex-ratio.          

 
Files available:

We provide 2 txt files: FLUCT_mating_fertility; FLUCT_productivity_sexratio.

 
FLUCT_mating_fertility: 

This file contains the raw data of male mating propensity and fertility under
four thermal conditions. 

Table headers are explained below: 

LineStatus - classification based on the reproductive performance
across three temperatures tested in Zwoinska et al. (2020)*. (High – lines whose
fertility did not decline as temperature increased; Low – lines whose fertility
declined substantially as temperature increased)

DGRP- line identity

Wolbachia- line infection status (y – infected; n- uninfected)

Haplotype- haplotypes identified in Bevers et al. (2019)*

Inv1/2/7/8/9 - the inversion status of each line for each of the
main inversions characterized in the DGRP (ST = standard, INV = inversion.
ST/INV = unfixed status in the line)

Temperature - mean developmental temperature experienced (25°C or
29°C)

ThermalRegime - thermal variation experienced (Const - constant or
Fluct - fluctuating regime)

Vial – vial where each pair was kept

EggCollDay – day the eggs were collected and placed in one of the
thermal conditions.

PairingDay – day males and females were paired

Mating – mating propensity (1, if mating observed; 0, if no mating
observed)

Larvae – fertility (1, if larvae present; 0, if no larvae present)

 
FLUCT_productivity_sexratio: 

This file contains the raw data of male productivity and sex ratio under
four thermal conditions. 

Table headers are explained below: 

LineStatus - classification based on the reproductive performance
across three temperatures tested in Zwoinska et al. 2020. (High - whose
fertility did not decline as temperature increased; Low - whose fertility
declined substantially as temperature increased)

DGRP- line identity

Wolbachia- line infection status (y – infected; n- uninfected)

Haplotype- haplotypes identified in Bevers et al. (2019)*

Inv1/2/7/8/9 - the inversion status of each line for each of the
main inversions characterized in the DGRP (ST = standard, INV = inversion.
ST/INV = unfixed status in the line)

Temperature - mean developmental temperature experienced (25°C or
29°C)

ThermalRegime - thermal variation experienced (Const - constant or
Fluct - fluctuating regime)

Vial – vial where each pair was kept

EggCollDay – day the eggs were collected and placed in one of the
thermal conditions.

PairingDay – day males and females were paired

Productivity – number of adult offspring produced.

Daughters – number of female offspring produced.

Sons – number of male offspring produced.

IGV – absolute deviation from the median as a measure of intra-genotypic
variability.

 
*Bevers, R. P. J., et al
(2019). Mitochondrial haplotypes affect metabolic phenotypes in the Drosophila
Genetic Reference Panel. Nature Metabolism, 1, 1226–1242. https://doi.org/10.1038/s42255-019-0147-3

Zwoinska, M. K.,
Rodrigues, L. R., Slate, J., &amp; Snook, R. R. (2020). Phenotypic responses to
and genetic architecture of sterility in response to sub-lethal temperature
during development. Frontiers in Genetics, 11, 573.
https://doi.org/10.3389/fgene.2020.00573</r:Content>
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