Supplementary Fig. 5c & 6b | Western blots of FUS and TARDBP in iPSC lysates
type: Dataset
distribution: Landing page for the dataset
description: This item is part of the Figshare Project: Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution (https://su.figshare.com/projects/Early_mitochondrial_dysfunction_revealed_across_FUS-_and_TARDBP-ALS_at_single_cell_resolution/163252) From Data Availability Statement for the forthcoming paper in Nature Communications entitled: Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS "We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study. [Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files." -------------------------------------------- [Item specific description:] This data belong to Supplementary Fig. 5 and 6. Western blots in iPSC lines with various FUS and TARDBP M337V mutations for FUS and TDP-43 protein expression. The assembled western blots are presented in the PPTX file. The fluorescent signal were scanned on a Odyssey CLx scanner (LI-COR) and exported as Image Studio ZIP archives. More information about the Image Studio software can be obtained here: https://www.licorbio.com/image-studio The raw images are given as TIF files, thumbnails as PNG, and aquisition meta information as TXT files within the exported archives.
modified: 2025-06-12T00:00:00Z
issued: 2025-04-28T00:00:00Z
publisher: Stockholm University
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Christoph Schweingruber
    type: Agent
    account: https://orcid.org/0000-0003-4505-9068
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Jonas Mechtersheimer
    type: Agent
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Marc-David Ruepp
    type: Agent
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Stefan Reber
    type: Agent
    type: PrivateIndividual(s)
keyword: Biochemistry and cell biology not elsewhere classified

Landing page for the dataset
type: Distribution
title: Landningssida för datasetet @sv
access URL: Supplementary Fig. 5c & 6b | Western blots of FUS and TARDBP in iPSC lysates
availability: stable

Stockholm University
type: Agent
name: Stockholms universitet @sv
type: Academia-ScientificOrganisation
source: 05f0yaq80