Data set for the Commun. Chem. article by Paul et al. with the title "13C- and 15N-labeling of amyloid-β and inhibitory polypeptides to study their interaction via nanoscale infrared spectroscopy"
type: Dataset
description: The data published here are the basis for the article by Paul et al. in Commun. Chem. with the title 13C- and 15N-labeling of amyloid-β and inhibitory polypeptides to study their interaction via nanoscale infrared spectroscopy and for the Research Square preprint by Paul et al. with DOI: 10.21203/rs.3.rs-2141341/v1 (https://doi.org/10.21203/rs.3.rs-2141341/v1) and title 13C-isotope-editing of nanoscale infrared images reveals the action of an inhibi­tory peptide against amyloid-β aggregation. These publications show that 13C, 15N-labeling can be used to discriminate between two peptides in nanoscale images of their infrared absorption, even when they have similar secondary structure. We studied different aggregation states of the amyloid-β peptide (Aβ) and its interaction with an inhibitory cell-penetrating peptide (NCAM1-PrP) using scattering-type scanning near-field optical microscopy (s-SNOM). Labeled and unlabeled peptides could be distinguished by comparing images of the optical phase taken at wavenumbers characteristic for either the labeled or the unlabeled peptide. Some of the provided files require particular software to view them: Gwyddion (http://gwyddion.net/) is needed for image files, neaPLOT (attocube) for nano-FTIR spectra, and OPUS (Bruker) for FTIR spectra. Processed neaPLOT files were stored as csv files. OPUS files were converted to txt files but can also be read by Spectragryph (https://www.effemm2.de/spectragryph/about.html), which is free for private and academic use.
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: Suman Paul
creator: Adéla Jeništová
creator: Faraz Vosough
creator: Elina Berntsson
creator: Cecilia Mörman
creator: Jüri Jarvet
creator: Astrid Gräslund
creator: Sebastian K. T. S. Wärmländer
creator: Andreas Barth
  type: Person
  affiliation: Stockholm University
  family name: Barth
  given name: Andreas
date modified: 2023-07-03
date published: 2023-07-03
keywords: Biological Sciences
  type: DefinedTerm
  term code: 106
keywords: Biochemistry
  type: DefinedTerm
  term code: 10408
keywords: Medical Biotechnology (Focus on Cell Biology, (incl. Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
  type: DefinedTerm
  term code: 30401
keywords: Medical biochemistry - proteins and peptides (incl. medical proteomics)
keywords: Analytical biochemistry
keywords: Molecular imaging (incl. electron microscopy and neutron diffraction)
keywords: Biological physics
keywords: Analytical spectrometry
license: https://creativecommons.org/licenses/by/4.0/
publisher: Stockholm University
version: 0

PropertyValue
property ID: DOI
value: 10.17045/STHLMUNI.23609580

Suman Paul
type: Person
family name: Paul
given name: Suman

Adéla Jeništová
type: Person
family name: Jeništová
given name: Adéla

Faraz Vosough
type: Person
family name: Vosough
given name: Faraz

Elina Berntsson
type: Person
family name: Berntsson
given name: Elina

Cecilia Mörman
type: Person
family name: Mörman
given name: Cecilia

Jüri Jarvet
type: Person
family name: Jarvet
given name: Jüri

Astrid Gräslund
type: Person
family name: Gräslund
given name: Astrid

Sebastian K. T. S. Wärmländer
type: Person
family name: Wärmländer
given name: Sebastian K. T. S.

Stockholm University
type: Organization