Fig. 3e-f | Quantitative immunofluorescence for TDP-43 localization in motor neuron lines
This item is part of the Figshare Project: Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution (https://su.figshare.com/projects/Early_mitochondrial_dysfunction_revealed_across_FUS-_and_TARDBP-ALS_at_single_cell_resolution/163252) From Data Availability Statement for the forthcoming paper in Nature Communications entitled: Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS "We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study. [Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files." -------------------------------------------- [Item specific description:] Immunofluorescence microscopy of TDP-43 in in-vitro differentiated ALS motor neurons. These files are related to quantification of TDP-43 localization and phosphorylation in ALS motor neurons using the Opera Phenix high content imaging system. More information here on our imaging platform: https://www.kclwcic.co.uk/operaphenix Sample meta information and plate layouts are available as XLSX and PDF files. The file format is .czi, through the ZEN software (Zeiss). Zeiss recommends using ImageJ and the ImageJ-based Fiji software package (https://imagej.net/software/fiji/) . Information about the microscopy file format CZI can be found here: https://www.zeiss.com/microscopy/en/products/software/zeiss-zen/czi-image-file-format.html