Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy
type: Dataset
distribution: Landing page for the dataset
description: This folder contains all raw data underlying the results presented in a manuscript, submitted for publication to Methods and Applications in Fluorescence, and entitled: Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy Authored by: Baris Demirbaya, Glib Baryshnikovb, Martin Haraldssonc, Joachim Pigueta, Hans Ågrend, Jerker Widengrena,* aRoyal Institute of Technology (KTH), Experimental Biomolecular Physics, Department of Applied Physics, Albanova University Center, SE-106 91, Stockholm, Sweden bLaboratory of Organic Electronics, Department of Science and Technology, Linköping University, SE-60174, Norrköping, Sweden cChemical Biology Consortium Sweden, Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE-171 77 Stockholm, Sweden dDepartment of Physics and Astronomy, Uppsala University, SE-751 20 Uppsala, Sweden *Corresponding Author: Email: jwideng@kth.se, Phone: +46-8-7907813 The data files are grouped into the different techniques used to generate them, and refer to the figures/tables in the manuscript where the extracted results are presented. ABSTRACT Photo-induced dark transient states of fluorophores can pose a problem in fluorescence spectroscopy, but their typically long lifetimes can also make them highly environment sensitive. This opens for exploiting these states as microenvironmental read-out parameters in bio-molecular spectroscopy and imaging, and to explore fluorophores with prominent dark-state formation yields to be used in such studies. In this work, we analyzed the singlet-triplet transitions of fluorescein by transient state (TRAST) spectroscopy and compared them with those of three synthesized carboxy-fluorescein derivatives, with one, two or four bromines linked to the anthracence backbone. By this bromination, a prominent internal heavy atom (IHA) enhancement of the intersystem crossing (ISC) rates was found, and a correspon…
modified: 2023-09-27T00:00:00Z
issued: 2023-05-12T00:00:00Z
publisher: Royal Institute of Technology
access rights: PUBLIC
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Demirbay Baris
    type: Agent
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Baryshnikov Glib
    type: Agent
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Haraldsson Martin
    type: Agent
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Ågren Hans
    type: Agent
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Piguet Joachim
    type: Agent
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Widengren Jerker
    type: Agent
    type: PrivateIndividual(s)
keyword: Fluorescence, fluorescein, bromination, triplet state, heavy atom effect, intersystem crossing

Landing page for the dataset
type: Distribution
title: Landningssida för datasetet @sv
access URL: Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy
availability: stable

Royal Institute of Technology
type: Agent
name: Kungliga Tekniska högskolan @sv
type: Academia-ScientificOrganisation
source: 026vcq606