Kungsängen soil microbial communities
type: Dataset
distribution: Landing page for the dataset
description: Since Linnaeus's time, the Kungsängen Nature Reserve has drawn frequent visitors because of its vegetation including the largest population of Fritillaria meleagris L. in Sweden. The reserve is a meadow with an abrupt moisture transition, where the wetter part has a Carex acuta dominated plant community with low species richness, visually distinct from the drier part, which has a species rich plant community including a high density of Fritillaria meleagris. To investigate the link between plant and below-ground micro-eukaryotic communities, we amplified and sequenced the ITS and LSU regions of the rDNA operon (1500 bp) from soil using PacBio SMRT sequencing, and evaluated three different methods for generation of operational taxonomic units. We found distinct communities associated with wet and mesic-dry soil conditions but estimated richness was not so different. Both soil conditions host communities dominated by protists, a large fraction of which were taxonomically assigned to Ciliphora (Alveolata), while 30-40% of all reads were assigned to kingdom Fungi. Ecological patterns were consistently recovered irrespective of the read clustering method used. However, different clustering methods affect the taxonomic and phylogenetic resolution of community characterization with implications for how well members of the micro-eukaryotic communities can be recognized in the data. This data resource contain amplicon sequence variant (ASV) sequences generated with DADA2 using the Ampliseq pipeline (https://nf-co.re/ampliseq). This dataset was published via the SBDI ASV portal (https://asv-portal.biodiversitydata.se/).
modified: 2026-02-19T00:00:00Z
issued: 2022-09-06T00:00:00Z
publisher: Uppsala University
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Anna Rosling
    type: Agent
    account: https://orcid.org/0000-0002-7003-5941
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Shadi Eshghi Sahraei
    type: Agent
    account: https://orcid.org/0000-0003-4741-5871
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Brendan Furneaux
    type: Agent
    account: https://orcid.org/0000-0003-3522-7363
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Kerri Kluting
    type: Agent
    account: https://orcid.org/0000-0002-2328-8081
    type: PrivateIndividual(s)
qualified attribution: Attribution
  had role: principalInvestigator
  agent: Mustafa Zakieh
    type: Agent
    type: PrivateIndividual(s)
keyword: Occurrence
keyword: OCCURRENCE
language: ENG

Landing page for the dataset
type: Distribution
title: Landningssida för datasetet @sv
access URL: Kungsängen soil microbial communities
availability: stable

Uppsala University
type: Agent
name: Uppsala universitet @sv
type: Academia-ScientificOrganisation
source: https://ror.org/048a87296