Kungsängen soil microbial communities
type: Dataset
description: Since Linnaeus's time, the Kungsängen Nature Reserve has drawn frequent visitors because of its vegetation including the largest population of Fritillaria meleagris L. in Sweden. The reserve is a meadow with an abrupt moisture transition, where the wetter part has a Carex acuta dominated plant community with low species richness, visually distinct from the drier part, which has a species rich plant community including a high density of Fritillaria meleagris. To investigate the link between plant and below-ground micro-eukaryotic communities, we amplified and sequenced the ITS and LSU regions of the rDNA operon (1500 bp) from soil using PacBio SMRT sequencing, and evaluated three different methods for generation of operational taxonomic units. We found distinct communities associated with wet and mesic-dry soil conditions but estimated richness was not so different. Both soil conditions host communities dominated by protists, a large fraction of which were taxonomically assigned to Ciliphora (Alveolata), while 30-40% of all reads were assigned to kingdom Fungi. Ecological patterns were consistently recovered irrespective of the read clustering method used. However, different clustering methods affect the taxonomic and phylogenetic resolution of community characterization with implications for how well members of the micro-eukaryotic communities can be recognized in the data. This data resource contain amplicon sequence variant (ASV) sequences generated with DADA2 using the Ampliseq pipeline (https://nf-co.re/ampliseq). This dataset was published via the SBDI ASV portal (https://asv-portal.biodiversitydata.se/).
contributor: Anna Rosling
contributor: Shadi Eshghi Sahraei
creative work status: completed
  type: DefinedTerm
  in defined term set: http://publications.europa.eu/resource/authority/dataset-status
  term code: COMPLETED
creator: Anna Rosling
creator: Shadi Eshghi Sahraei
creator: Brendan Furneaux
creator: Kerri Kluting
creator: Mustafa Zakieh
  type: Person
  affiliation: Department of Ecology and Genetics, Uppsala University
date modified: 2026-02-19
date published: 2022-09-06
language: English
keywords: Biological Sciences
  type: DefinedTerm
  term code: 106
keywords: Occurrence
keywords: OCCURRENCE
license: https://creativecommons.org/publicdomain/zero/1.0/
publisher: Department of Ecology and Genetics, Uppsala University
version: 0

PropertyValue
property ID: DOI
value: 10.15468/UPXBWY

PropertyValue
property ID: UUID

Anna Rosling
type: Person
affiliation: Department of Ecology and Genetics, Uppsala University

Department of Ecology and Genetics, Uppsala University
type: Organization
name: Uppsala University

Shadi Eshghi Sahraei
type: Person
affiliation: Department of Ecology and Genetics, Uppsala University

Brendan Furneaux
type: Person
affiliation: Department of Ecology and Genetics, Uppsala University

Kerri Kluting
type: Person
affiliation: Department of Ecology and Genetics, Uppsala University

English
type: Language
alternate name: eng