Kaffe, homocystein och B-vitaminer Coffee, homocysteine and B vitamins snd0015-1-1 Swedish National Data Service Svensk nationell datatjänst http://swepub.kb.se/bib/swepub:oai:gup.ub.gu.se/116082 http://gup.ub.gu.se/publication/116082-coffee-and-gene-interaction-the-effect-on-methionine-and-lipid-metabolism http://swepub.kb.se/bib/swepub:oai:gup.ub.gu.se/51032 http://gup.ub.gu.se/publication/51032-the-methylenetetrahydrofolate-reductase-c677t-polymorphism-is-a-major-determinant-of-coffee-induced Kaffe, homocystein och B-vitaminer Coffee, homocysteine and B vitamins snd0015-1-1 91-628-6022-4 Strandhagen, Elisabeth Strandhagen, Elisabeth Zetterberg, Henrik Zetterberg, Henrik Swedish National Data Service Svensk nationell datatjänst http://swepub.kb.se/bib/swepub:oai:gup.ub.gu.se/116082 http://gup.ub.gu.se/publication/116082-coffee-and-gene-interaction-the-effect-on-methionine-and-lipid-metabolism http://swepub.kb.se/bib/swepub:oai:gup.ub.gu.se/51032 http://gup.ub.gu.se/publication/51032-the-methylenetetrahydrofolate-reductase-c677t-polymorphism-is-a-major-determinant-of-coffee-induced Blood Pressure Blodtryck Blood Specimen Collection Blodprovstagning Homocysteine Homocystein Alanine Transaminase Alanintransaminas Aspartate Aminotransferases Aspartataminotransferaser Folic Acid Folsyra gamma-Glutamyltransferase Gammaglutamyltransferas Genes Gener Cardiovascular Diseases Kardiovaskulära sjukdomar Coffee Kaffe Cholesterol Kolesterol Dietary Supplements Kosttillskott Body Height Kroppslängd Body Weight Kroppsvikt Nutritional Sciences Näringsvetenskap Placebos Placebo Smoking Rökning Triglycerides Triglycerider Uric Acid Urinsyra Vitamin B Complex B-vitamin komplex HEALTH HÄLSA Specific diseases, disorders and medical conditions Specifika sjukdomar, störningar och medicinska tillstånd Elevated level of homocysteine has been identified as a graded and independent risk factor for coronary heart disease. The metabolism of homocysteine is dependent on the availability of the B-vitamins, folate, vitamin B12, and vitamin B6 (pyridoxal phosphate). Although elevated levels of tHcy in plasma and serum is particularly seen in folate deficiency, elevated levels are also seen in the presence of folate levels in the lower reference range. The study was organised as a prospective, cross-over, blinded controlled trial with the participants randomly allocated to two different groups which will consume: 1) For 3 weeks: Both groups: no coffee 2) For 4 weeks: Group A: 4 cups of coffee/day + placebo, Group B: 4 cups of coffee/day + 200 g folate/day 3) For 3 weeks: Both groups: no coffee (wash out) 4) For 4 weeks: Group A. 4 cups of coffee/day + 40 g B-6/day, Group B: 4 cups of coffee/day + placebo. The participants were randomised again in the beginning of the second part. Each participant met at five visits. They were allowed to drink other beverages. Diet habits were registered during the study. Blood samples were drawn and blood pressure measured at every visit. Blood samples were analysed for homocysteine in plasma and total cholesterol, HDL-cholesterol, triglycerides, Lp(a), folate, ALAT, ASAT, urate and gamma-GT in serum. Frosen whole blood is available. Purpose: To investigate the interaction of coffee consumption on the effects of folate and vitamin B-6 on the methionin metabolism and thereby on the plasma homocysteine levels. Studien gjordes som ett doktorandprojekt vid Göteborgs Universitet med bakgrund i att förhöjd nivå av homocystein har satts i samband med ökt risk för hjärt-kärl-sjukdom. De viktigaste faktorerna som höjer homocysteinnivån i blodet är lågt intag av B-vitaminer (framförallt folsyra), rökning och kaffe. En randomiserad placebo kontrollerad interventionstudie gjordes för att se om supplementering med folat (delstudie 1) eller vitamin B6 (delstudie 2) kunde eliminera homocysteinökningen som uppkommer av normala doser kaffe. Studien genomfördes som två prospektiva, randomiserade, dubbelblinda kontrollerade försök. Försökspersonerna randomiserades till två grupper enligt följande: 1) Under 3 veckor: Båda grupperna: inget kaffe 2) Under 4 veckor: Grupp A: 6 dl kaffe/dygn + 200 mikrog folat/dygn, Grupp B: 6 dl kaffe/dygn + placebo 3) Under 3 veckor: Båda grupperna: inget kaffe 4) Under 4 veckor: Grupp A: 6 dl kaffe/dygn + 40 mg vitB6/dygn, Grupp B: 6 dl kaffe/dygn + placebo. Vid början av delstudie 2 randomiserades deltagarna på nytt. Vid första besöket genomfördes en kostundersökning med speciell inriktning på folat- och vitB6-innehållande livsmedel. Kostvanorna registrerades även under studiens gång. Blodprov och blodtryck togs vid alla 5 tillfällen. Under studien drack försökspersonerna endast den mängd kaffe som anges ovan. Eventuella avvik registrerades. Andra drycker fick konsumeras fritt. Blodproverna som togs vid varje besökstillfälle analyserades avseende homocystein i plasma och total kolesterol, HDL-kolesterol, triglycerider, Lp(a), folat, ASAT, ALAT, urat och gamma-GT i serum. Helblod finns fryst. Syfte: Att studera interaktionen mellan kaffekonsumtion och effekten av folat och vitamin B6 på metioninmetabolismen och därigenom effekten på plasma homocystein. 2000-10 2001-04 Sweden Sverige IndividualIndividual IndividIndivid The participants were in the age-range 35-69 years and usual coffee drinkers, free of clinically recognised chronic diseases such as cardiovascular diseases, cancer, renal disorders, liver disease, diabetes mellitus, and were not on anti-epileptic or chol Deltagarna var 35-69 år, hade använt kaffe regelbundet under de senaste 5 åren, var friska avseende hjärtkärl sjukdom, cancer, njursjukdom, lever sjukdom, diabetes och stod inte på regelbunden antiepileptisk eller lipidsänkande medicinering. Numeric The participants were recruited by advertisement in daily press.The participants were recruited by advertisement in daily press. Försökspersonerna rekryterades via annonser i dagspressen.Försökspersonerna rekryterades via annonser i dagspressen. Non-probability: AvailabilityNon-probability: Availability Icke-sannolikhetsurval: tillgänglighetsurvalIcke-sannolikhetsurval: tillgänglighetsurval Access to data through an external actor. Access to data is restricted. Åtkomst till data via extern aktör. Tillgång till data är begränsad. restrictedAccess Strandhagen E. Coffee and gene interaction. The effect on methionine and lipid metabolism. Thesis, University of Gothenburg, 2004. Strandhagen E. Coffee and gene interaction. The effect on methionine and lipid metabolism. Thesis, University of Gothenburg, 2004. 91-628-6022-4 2004 Strandhagen E, Landaas S, Thelle DS. Folate supplement eliminates the homocysteine increasing effect of filtered coffee. A randomised placebo controlled trial. Eur J Clin Nutr, 2003; 57: 1411-1417 Strandhagen E, Landaas S, Thelle DS. Folate supplement eliminates the homocysteine increasing effect of filtered coffee. A randomised placebo controlled trial. Eur J Clin Nutr, 2003; 57: 1411-1417 2003 Strandhagen E, Zetterberg H, Aires A, Palmér M, Rymo L, Blennow K, Thelle DS. The apolipoprotein E genetic polymorphism and the cholesterol raising effect of coffee. Lipids in Health and Disease 2004; 3: 26 (30Nov2004) Strandhagen E, Zetterberg H, Aires A, Palmér M, Rymo L, Blennow K, Thelle DS. The apolipoprotein E genetic polymorphism and the cholesterol raising effect of coffee. Lipids in Health and Disease 2004; 3: 26 (30Nov2004) 2004 Strandhagen E, Thelle DS. Filtered coffee raises serum cholesterol. Results from a controlled intervention trial. Eur J Clin Nutr, 2003; 57: 1164-1168. Strandhagen E, Thelle DS. Filtered coffee raises serum cholesterol. Results from a controlled intervention trial. Eur J Clin Nutr, 2003; 57: 1164-1168. 2003 Strandhagen E, Zetterberg H, Aires N, Palmér M, Rymo L, Blennow K, Landaas S, Thelle DS. The methylenetetrahydrofolate reductase C677T polymorphism is a major determinant of coffee-induced increase of plasma homocysteine: a randomized placebo controlled study. Int J Mol Med 2004: 6: 811-5. Strandhagen E, Zetterberg H, Aires N, Palmér M, Rymo L, Blennow K, Landaas S, Thelle DS. The methylenetetrahydrofolate reductase C677T polymorphism is a major determinant of coffee-induced increase of plasma homocysteine: a randomized placebo controlled study. Int J Mol Med 2004: 6: 811-5. 2004