https://researchdata.se/en/catalogue Search results en This dataset contains IsoSeq transcripts, sequence repeat annotations, and estimates of genetic diversity and Tajima’s D across the genomes of three Anthophora bee species: A. quadrimaculata, A. retusa and A. plagiata. A brief description of each file type is provided below. A detailed explanation of the methods used to generate the files can be found in the publication available via https://doi.org/10.1111/mec.70204. IsoSeq transcripts:PacBio IsoSeq transcripts aligned to the primary genome assembly using miniprot v.0.10-r226. The raw IsoSeq data are deposited in the European Nucleotide Archive (ENA) under the accession numbers: - A. quadrimaculata (iyAntQuad1), PRJEB72355 - A. plagiata (iyAntPlag1), PRJEB72356 - A. retusa (iyAntRet1), PRJEB72357 Files: - Anthophora_plagiata_isoseq.gff - Anthophora_retusa_isoseq.gff - Anthophora_quadrimaculata_isoseq.gff Repeats:Nucleotide sequences of the predicted repetitive elements in GFF format. Repeats were identified using RepeatModeler2. Files: - Anthophora_plagiata_repeats.gff - Anthophora_retusa_repeats.gff - Anthophora_quadrimaculata_repeats.gff Nucleotide diversity Pi:Nucleotide diversity (π) was estimated in non-overlapping 5 kb windows across each genome using variant calls generated from population sequencing data from 57, 52, and 27 individuals for A. retusa, A. quadrimaculata, and A. plagiata, respectively. This estimate was calculated using vcftools v. 0.1.17. π reflects the average number of nucleotide differences per site between two randomly chosen sequences. Files: - plag_5kb.windowed.pi - quad_5kb.windowed.pi - retusa_5kb.windowed.pi Tajimas’D:Tajima’s D was calculated in sliding windows across the genome using SNP datasets for each species using vcftools v. 0.1.17. Tajima’s D summarizes allele frequency spectra and can indicate departures from neutral evolution, such as population expansion, contraction, or selection. Files: - plagiata.Tajima.D.end_column_5kb_midpoint.bed - quadri.Tajima.D.end_column_5kb_midpoint.bed - retusa.Tajima.D.end_column_5kb_midpoint.drop_nan.bed Available variables:For a description of the column headers in the GFF file format, please refer to the respective documentation: https://github.com/The-Sequence-Ontology/Specifications/blob/master/gff3.md Mon, 12 Jan 2026 00:00:00 GMT https://researchdata.se/en/catalogue/dataset/doi-10-17044-scilifelab-30884846 https://researchdata.se/en/catalogue/dataset/doi-10-17044-scilifelab-30884846 Uppsala University Demetris Taliadoros André E. R. Soares Guilherme Borges Dias Ignas Bunikis Martin Pippel Anna Olsson Mai-Britt Mosbech Julia Heintz Nina Lager Anna-Sofi Strand Mats Pettersson Olga Vinnere Pettersson Henrik Lantz Björn Cederberg Kerstin Lindblad-Toh Matthew T. Webster