https://researchdata.se/en/catalogue Search results en Pilot short-read genome assembly of one single adult barnacle, Balanus improvisus (=Amphibalanus imrpovisus), used to estimate genome size and heterocygosity. Bi01.clean.fa.gz is the final assembly with contaminations etc removed. Alm Rosenblad et al., Marine Biotechnology 2021 Abstract Barnacles are key marine crustaceans in several habitats and they constitute a common practical problem by causing biofouling on man-made marine constructions and ships. Despite causing considerable ecological and economic impacts, there is a surprising void of basic genomic knowledge, and a barnacle reference genome is lacking. We here set out to characterize the genome of the bay barnacle Balanus improvisus (= Amphibalanus improvisus) based on short-read whole genome sequencing and experimental genome size estimation. We show both experimentally (DNA staining and flow cytometry) and computationally (k-mer analysis) that B. improvisus has a haploid genome size of ~ 740 Mbp. A pilot genome assembly rendered a total assembly size of ~600 Mbp and was highly fragmented with an N50 of only 2.2 kbp. Further assembly-based and assembly-free analyses revealed that the very limited assembly contiguity is due to the B. improvisus genome having an extremely high nucleotide diversity (π) in coding regions (average π ≈ 5% and average π in 4-fold degenerate sites ≈ 20%), and an overall high repeat content (at least 40%). We also report on high variation in the α-octopamine receptor OctA (average π = 3.6%), %), which might increase the risk that barnacle populations evolve resistance towards antifouling agents. The genomic features described here can help in planning for a future high-quality reference genome, which is urgently needed to properly explore and understand proteins of interest in barnacle biology and marine biotechnology and for developing better antifouling strategies. Fri, 16 Apr 2021 00:00:00 GMT https://researchdata.se/en/catalogue/dataset/doi-10-17044-scilifelab-14339153 https://researchdata.se/en/catalogue/dataset/doi-10-17044-scilifelab-14339153 University of Gothenburg Magnus Alm Rosenblad Björn Nystedt Supplementary tables for the Review article "Connectivity and Population Structure in a Marginal Sea - a Review" (https://doi.org/10.1111/ddi.70056). Contains information on connectivity and population structure within marine species inhabiting the Skagerrak sea, summarised from scientific studies published between the years 1990 and 2023. Table S1: List of all 484 scientific studies that were screened, as well as information regarding whether they were included in the literature review. Table S2: Contains extracted data from the 172 scientific studies, published between 1990 and 2023, that were reviewed with regard to connectivity and population structure in the Skagerrak. The data includes information about both the studies' methodology (such as scientific discipline, spatial and temporal scale, and sample size) and results (such as which populations were identified and estimated connectivity between different regions). Table S3: Detailed summary of the scientific studies that studied contemporary connectivity on a "large" scale (between the Skagerrak sea and adjacent seas). For species with information from several studies, a concensus result for the species was created. Table S4: Detailed summary of the scientific studies that studied contemporary connectivity on a "small" scale (within the Skagerrak sea). For species with information from several studies, a concensus result for the species was created. Table S5: Detailed summary of the scientific studies that studied population structure, both within the Skagerrak Sea and between the Skagerrak and adjacent seas. For species with information from several studies, a concensus result for the species was created. Tables S6-S7: Settings for the BARRIER analysis conducted in the study. For more detailed descriptions of the content we refer to the text in the original study. This dataset is listed as "Deliverable 2.2" of the MARHAB project (Horizon Europe, grant number 101135307). Mon, 10 Nov 2025 12:52:33 GMT https://researchdata.se/en/catalogue/dataset/2025-294 https://researchdata.se/en/catalogue/dataset/2025-294 University of Gothenburg Simon Henriksson Per Erik Jorde Charlotte Berkström Guldborg Søvik Pierre De Wit Halvor Knutsen Even Moland Carl André Marlene Jahnke