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      <title>Data on how honeybee host brood traits influence Varroa destructor reproduction</title>
      <description>The data set was collected in Uppsala Sweden between 2019 and 2021. Hives were established using varroa resistant queens from Oslo, Norway (n = 3), Gotland Sweden, (n = 5), and Avignon, France (n = 4), with a varroa susceptible population from Uppsala, Sweden (n = 5) as control. All hives were located at the SLU Lövsta research station (GPS Coordinates: 59° 50’ 2.544”N, 17° 48’ 47.447”E). Varroa destructor mite reproductive success was measured on frames with adult honeybee workers exposed to, and excluded from access to honeybee larvae. Excluders were added directly after brood capping, and frames were dissected nine days later. Cell caps were removed using a scalpel with the pupae and mite families carefully removed from the cell using forceps and a fine paint brush. Mite reproductive success calculated by counting successful reproduction attempts, which was defined as a mite that successfully produced one male, and at least one female offspring. If a mite did not meet this requirement, it was considered a failed reproduction attempt and the reason for failure was documented. All data was analyzed in R version 4.0.1 using R Studio 1.3.959. A linear mixed-effect model was used with mite reproductive success as the response variable, population origin and excluder treatment as independent variables, with colony and year as random effect variables to compare treatments within each population as well as fecundity. Least-square means of the model were used to compare treatments between individual populations.

Scaramella_et_al_2023_Data.tsv - Data set consists of 34 rows and 21 columns. Colony demographics, and designated treatment are listed. All data collected are count data and are explained in more detail in read me file. 
R script used in analysis is attached. It is split into two sections, with the first being used for statistical analysis, and the second used for plot creations used in the paper. Sections defined by title SECTION 1 - ANALYSIS and SECTION 2 - PLOTs

The  output Scaramella_et_al_2023_Analysis_Code_log.txt and plot file Rplots.pdf can, provided that the script is in the same directory as the data files and needed R packages are installed (see sessionInfo.txt), be reproduced by running:
Rscript Scaramella_et_al_2023_Analysis_Code.R &gt; \
Scaramella_et_al_2023_Analysis_Code_log.txt

Scaramella_et_al_2023_Bar_Graph_Data.tsv - Data set consisting of 8 rows &amp; 5 columns. Colony demographics, and designated treatment are listed. All data generated from the count data in Scaramella_et_al_2023_Data.tsv and are explained in more detail in read me file.

Scaramella_et_al_2023_Stacked_Bar_Graph_Data.tsv - Data set consisting of 102 rows &amp; 8 columns. Colony demographics, and designated treatment are listed. All data is Scaramella_et_al_2023_Data.tsv restructured to include reason failed as a column. The data is explained in more detail in read me file.</description>
      <pubDate>Thu, 01 Jun 2023 11:09:57 GMT</pubDate>
      <link>https://researchdata.se/en/catalogue/dataset/2023-79-1</link>
      <guid>https://researchdata.se/en/catalogue/dataset/2023-79-1</guid>
      <dc:publisher>Swedish University of Agricultural Sciences</dc:publisher>
      <dc:creator>Nicholas Scaramella</dc:creator>
      <dc:creator>Ashley Burke</dc:creator>
      <dc:creator>Melissa Oddie</dc:creator>
      <dc:creator>Barbara Locke</dc:creator>
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      <title>Unique brood ester profile in a Varroa destructor resistant population of European honey bee (Apis mellifera)</title>
      <description>Data of chemical compounds extracted from honey bee (Apis mellifera) brood in a varroa resistant and varroa susceptible population. Samples were taken 0, 6, 12, 18, 24, and 36 hours after brood capping by immersing pupae in pentane for 10 minutes. 6 hives from each population were used, and 3 replicates were taken per hive per time point. The FAME column was calculated by adding Methyl Palmitate, Linoloate, and Stearate together, while the FAEE columb was calculateed by adding Ethyl Palmitate, Linoloate, and Stearate together. Samples were analyzed using gas chromatography. 

R version 4.4.0 and RStudio version 1.4.1.748 were used to analyze the data. All packages and their version is listed in the attached R script. 

Data files included:
Scaramella_et_al_2024_Contrast.tsv: 16 rows × 12 columns
Scaramella_et_al_2024_Data.tsv: 210 rows × 14 columns</description>
      <pubDate>Mon, 24 Jun 2024 13:15:10 GMT</pubDate>
      <link>https://researchdata.se/en/catalogue/dataset/2024-173</link>
      <guid>https://researchdata.se/en/catalogue/dataset/2024-173</guid>
      <dc:publisher>Swedish University of Agricultural Sciences</dc:publisher>
      <dc:creator>Nicholas Scaramella</dc:creator>
      <dc:creator>Barbara Locke</dc:creator>
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