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      <title>Greiser et al. (2025) Bark beetles as microclimate engineers – thermal characteristics of infested spruce trees at the canopy surface and below the canopy - Data and code</title>
      <description>Two R-scripts and data are provided to generate the statistical models and plots.



Related publication - abstract:

Over recent decades, Spruce bark beetle outbreaks have expanded and intensified across Europe, driven by a warming climate and more frequent extreme drought events. While research has largely focused on early detection and vulnerability prediction, little is known about the consequences of beetle infestations on forest microclimates. Bark beetle attacks are expected to alter forest microclimates due to changes in canopy cover, albedo, wind patterns, and evapotranspiration. We explored the effect of bark beetle attacks on summer forest microclimate using two approaches.

Firstly, we measured understory microclimate at 2-m height in 31 Swedish forest stands using small consumer-grade loggers. Along a gradient of attack severity, represented by increasing proportions of dead spruces, maximum summer day-time temperatures increased by up to 2 °C, with this warming effect being moderated by the presence of deciduous broadleaf trees. Surprisingly, night-time minimum temperatures were not affected by bark beetle attacks.

Secondly, we mapped canopy surface temperature over one part of the study area using multispectral and thermal drone imaging, contrasting canopy temperatures of healthy and infested trees. We observed that dead trees were generally warmer than living trees, by an average of 2.6 °C on a sunny day and 0.7 °C on a cloudy day.

Our study documented changes in thermal regimes in both the understory and overstory after bark beetle attacks, indicating that climate-change related disturbances are fueling rapid increases in microclimate warming. However, even dead forest stands may function as thermal buffers for understory vegetation, as we found minimum temperatures being unaffected by bark beetles. Finally, our results suggest that increasing the proportion of deciduous trees can decrease the risk of bark-beetle induced microclimate warming. The insights gained can guide forest succession and regeneration management after disturbances, contributing to critical decisions on conservation areas and salvage logging strategies.</description>
      <pubDate>Tue, 19 Aug 2025 00:00:00 GMT</pubDate>
      <link>https://researchdata.se/sv/catalogue/dataset/doi-10-17045-sthlmuni-28615514</link>
      <guid>https://researchdata.se/sv/catalogue/dataset/doi-10-17045-sthlmuni-28615514</guid>
      <dc:publisher>Stockholms universitet</dc:publisher>
      <dc:creator>Caroline Greiser</dc:creator>
      <dc:creator>Koenraad Van Meerbeek</dc:creator>
      <dc:creator>Christophe Metsu</dc:creator>
      <dc:creator>Langning Huo</dc:creator>
      <dc:creator>Ian brown</dc:creator>
      <dc:creator>Mark Ghaly</dc:creator>
      <dc:creator>Philipp Lehmann</dc:creator>
    </item>
    <item>
      <title>Whole genome analyses based on single, field collected spores of the AMF F.geosporum</title>
      <description>The item contains data and figures supporting the publication with the same name. In this study we sequenced nuclei and assembled genomes for two spores from an arbuscular mycorrhizal fungi morphotypically identified as Funneliforms geosporum. The spores were collected at the Kunsgängen nature reserve just south of Uppsala, Sweden.

The file "Spore nuclie scatter plots and MDA results for ..." contains information on the spores from which nuclei were sorted in this project including FACS scatterplot, well positions, MDA, PCR screening for bacteria and fungi as well as a list of which nuclei were selected for sequening. 

The zipped folder "Alignments for species identification.zip" contains alignments and phylogenetic trees for there reference genes (Fox, LSU and Tor) used for species identification of the morphotyped spores agains known species.

The file "Alignment of MAT contigs from all funneliformis.phy" contains one contig from each of the four Funneliformis genome assemblies with a gene homologous to one of the genes in the mating type locus identified in Rhizophagus irregulars, gene bank accession number KT946687

The file "Alignment of MAT contigs from all funneliformis extraction.fasta" contains the region from each of the four contigs that was extracted from the above alignment and used to Blast all single nuclei assemblies for potential mating type locus homologues.

The two excel files "Fig 3a..." and "Fig 3b..." contains the FunAnnotate output for gene families CAZyme and MEROPS and calculations for Fig 3a and b.</description>
      <pubDate>Mon, 12 Sep 2022 00:00:00 GMT</pubDate>
      <link>https://researchdata.se/sv/catalogue/dataset/doi-10-17044-scilifelab-20120150</link>
      <guid>https://researchdata.se/sv/catalogue/dataset/doi-10-17044-scilifelab-20120150</guid>
      <dc:publisher>Uppsala universitet</dc:publisher>
      <dc:creator>Shadi Eshghi Sahraei</dc:creator>
      <dc:creator>Marisol Sanchez Garcia</dc:creator>
      <dc:creator>Merce Montoliu-Nerin</dc:creator>
      <dc:creator>David Manyara</dc:creator>
      <dc:creator>Claudia Bergin</dc:creator>
      <dc:creator>Søren Rosendahl</dc:creator>
      <dc:creator>Anna Rosling</dc:creator>
    </item>
    <item>
      <title>Forest Fire Influence on Tomicus piniperda-Associated Fungal Communities and Phloem Nutrient Availability of Colonized Pinus sylvestris</title>
      <description>The study presents results of fungal metabarcoding community data from bark beetles and phloeme collected in burned and unburned forest sites in central Sweden and phloem chemistry data.

The folder "tomicus_2021" contains data and scripts used to perform statistical analysis and generate figures presented in the paper "Fire Influence on Tomicus piniperda-Associated Fungal Communities and Phloem Nutrient Availability of Colonized Pinus sylvestris" and its supplementary material. 

The folder "Corrected Tables" contains excel files with all the final tables presetned in the paper and the supplementary materail.

The files pb_466_001 and 002 contain FASTQ files from the pacbio run. These can be demultiplexed with the two primer files (Primer-ITS4_Tag-setup.txt and Primer_fITS9_Tag-setup.txt) that contain info on the tag sequence for each tag used.</description>
      <pubDate>Wed, 24 Aug 2022 00:00:00 GMT</pubDate>
      <link>https://researchdata.se/sv/catalogue/dataset/doi-10-17044-scilifelab-20289000</link>
      <guid>https://researchdata.se/sv/catalogue/dataset/doi-10-17044-scilifelab-20289000</guid>
      <dc:publisher>Uppsala universitet</dc:publisher>
      <dc:creator>Anna Rosling</dc:creator>
      <dc:creator>Kerri Kluting</dc:creator>
      <dc:creator>Diana six</dc:creator>
      <dc:creator>Ylva Strid</dc:creator>
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