https://researchdata.se/sv/catalogue Search results sv This item is part of the Figshare Project: Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution (https://su.figshare.com/projects/Early_mitochondrial_dysfunction_revealed_across_FUS-_and_TARDBP-ALS_at_single_cell_resolution/163252) From Data Availability Statement for the paper in Nature Communications entitled: Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS "We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study. [Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files." -------------------------------------------- [Item specific description:] Quantitative immunofluorescence microscopy was used to assess the expression of mitochondrial genes MT-CO1, MT-CO2, MT-CO3, and NDUFA12 in ALS motor neuron axons. These files are confocal images of immunofluorescent stainings of mitochondrial proteins in iPSC-derived motor neurons. The file format is .czi, through the ZEN software (Zeiss). Zeiss recommends using ImageJ and the ImageJ-based Fiji software package (https://imagej.net/software/fiji/) . Information about the file format CZI can be found here: https://www.zeiss.com/microscopy/en/products/software/zeiss-zen/czi-image-file-format.html Mon, 28 Apr 2025 00:00:00 GMT https://researchdata.se/sv/catalogue/dataset/doi-10-17045-sthlmuni-27939792 https://researchdata.se/sv/catalogue/dataset/doi-10-17045-sthlmuni-27939792 Stockholms universitet Christoph Schweingruber Mélanie Leboeuf This item is part of the Figshare Project: Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution From Data Availability Statement for the paper in Nature Communications entitled: Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS "We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study. [Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files." ------------------------- [Item specific description:] Quantititative fluorescene microscopy for FUS protein in ALS motor neurons The dataset are Harmony archives for the high content imager Opera Phenix (Perkim Elmer). The proprietary harmony software creates a database with file organization stored in xml and oar files and folders with raw tif files. More information here on our imaging platform: https://www.kclwcic.co.uk/operaphenix These archives contain thousands of tif files. Therefore, they are compressed into tar.gz files with matching md5 checksums for verification. The prism file is just a graphpad prism file with the summary data. More info here: https://www.graphpad.com/features Thu, 10 Apr 2025 00:00:00 GMT https://researchdata.se/sv/catalogue/dataset/doi-10-17045-sthlmuni-27952080 https://researchdata.se/sv/catalogue/dataset/doi-10-17045-sthlmuni-27952080 Stockholms universitet Christoph Schweingruber Erin Hedges Marc-David Ruepp