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Data from various stunning attempts for Nile tilapia (Oreochromis Niloticus)

https://doi.org/10.5878/59q2-7h64
The study that this data supports aimed to evaluate the stunning effectiveness of various stunning attempts on Nile tilapia by measuring brain activity to assess neurological indicators of conscioussness/unconsciousness. Thus, this data set contains raw data from measurements of brain activity using electroencephalography in Nile tilapia. Data were collected before and after stunning attempts using the following stunning methods: Live chilling, mechanical stunning (percussion), electrical stunning and a combinational approach consisting of electrical stunning followed by direct throat cutting and chilling. The data is extracted from Labchart, where data was collected using a Powerlab and a Bioamp. The data contains the mean amplitude during 20-80 s during the 200 ms long light and dark phases. The data set also contains the mean amplitude during 10 s before short electrical exposure as well as the mean amplitude of the signal during the time that the epileptic-like seizure lasted after electrical exposure (i.e., 9-27 s). The data also include information on the time that visually evoked responses (VERs) were lost after stunning, as well as the induction time (when applicable) for the loss of VERs.
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Data files

Documentation files

Citation and access

Data access level:

Creator/​Principal investigator(s):

Research principal:

Principal's reference number:

  • SLU.thv.2024.4.4-105-1

Data contains personal data:

No

Citation:

Language:

Method and outcome

Unit of analysis:

Population:

Nile tilapia (Oreochromis niloticus)

Study design:

  • Observationsstudie
  • Experimentell studie

Description of study design:

Brain activity was measured using needle electrodes that were placed near the brain by penetrating the skin and underlying tissues. Electroencephalography (EEG) was used as a method to study how the fish responded to different stunning methods. That is, the baseline EEG of the fish was collected before any stunning was applied, and the fish then underwent one of four different stunning protocols. These were i) mechanical stunning by shooting the fish on the head with a non-penetrating bolt gun driven by pressurized air, ii) electrical stunning where both short electrical stunning (1 s) and longer electrical stunning (30 s) were tested, iii) live chilling where the fish was rapidly cooled down by adding ice cold water to tank, replacing the warm water immersing the fish iv) a combinational approach where electrical stunning for 30 s was followed by direct throat cutting and cooling. Before, during and after the stunning procedure, the fish were observed to note differences and/or deviations in behavior and physiological expressions (for example ventilation) and the EEG was measured.

Time period(s) investigated:

Data format/​data structure:

Species and taxons:

Data collection Laboratorieexperiment

Mode of collection:

Laboratorieexperiment

Description of the mode of collection:

EEG samlades in med hjälp av elektroder som plockade upp den elektriska aktiviteten i hjärnan. Genom att stimulera fisken med ett repetitivt visuellt stimuli kunde responser framkallas om fisken var vid medvetande, medan dessa responser på ljuset saknades om fisken var medvetslös. De repetitiva stimuli som användes här var en lampa som tändes och släcktes med en frekvens av 2 Hz vars varaktighet (dvs tiden som lampan var tänd) var 5 ms per gång. För att ge förutsättningar att stimuleras av lampan hölls rummet i övrigt mörkt. Flera mätningar (mellan xx-yy stycken) överlappades för att generera medelresponsen under den angivna tiden. Responsen delades i sin tur upp i två delar som vardera var 200 ms långa, den 200 ms långa ljusa fasen (vilken kretsade kring de 5 ms av tänt ljus) och den 200 ms långa mörka fasen som följde den 200 ms ljusa fasen (och alltså skedde innan dess att lampan tändes i 5 ms igen). Datan samlades in med hjälp av ett Powerlab och signalen amplifierades av en BioAmp. Datan kunde sedan extraheras i programmet Labchart och överfördes till excel där uträkningar gjordes.

Time period(s) for data collection:

2021-01-31 - 2021-04-30

Data collector:

Instrument

Name:

Elektroder

Description of the instrument:

21 gauge nålar löddes på silvertrådar från AD instrument för att fungera som elektroder och plocka upp den elektriska aktiviteten i hjärnan.

Name:

PowerLab

Description of the instrument:

Ett 16 SP PowerLab från AD instruments användes för att samla in EEG signalerna samt de signaler som solpanelen skickade när den stimulerats av ljus.-

Name:

Elbedövare för elbedövning i vatten

Description of the instrument:

En bedövningsmaskin för elektrisk bedövning i vatten tillverkad av Ace Aquatec användes. Maskinen hade förmågan att leverera 50 Hz AC ström.

Name:

Bio förstärkare

Description of the instrument:

En förstärkare från AD instruments användes för att förstärka EEG signalen

Administrative information

Responsible department/​unit:

Departement of Applied Animal Science and Welfare

Contributor(s):

Commissioning organisation:

  • Institutionen för Biologi och miljövetenskap

Registration number at commissioning organization:

  • Institutionen för Biologi och miljövetenskap

Ethical Review

Reviewer:

  • Göteborgs etikprövningsnämnd

Registration number:

5.8.18-12466/2018

Funding

Funding agency:

Award number:

2021-01556

Funding agency:

Funding agency:

Award number:

2021-02303

Topic and keywords

Swedish Standard Classification of Research Subjects 2025:

Keywords:

Publications

Citation:

Sundell, Erika & Brijs, Jeroen & Gräns, Albin. (2024). The quest for a humane protocol for stunning and killing Nile tilapia (Oreochromis niloticus). Aquaculture. 593. 741317.

Contact

SLU Arkivarkiv@slu.se

Metadata

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