Data from various stunning attempts for Nile tilapia (Oreochromis Niloticus)

• Erika Sundell - Swedish University of Agricultural Sciences - Department of Applied Animal Science and Welfare

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• Swedish University of Agricultural Sciences

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• SLU.thv.2024.4.4-105-1

• Data are freely accessible

• English

• Animal

Nile tilapia (Oreochromis niloticus)

• Observational study
• Experimental study

Brain activity was measured using needle electrodes that were placed near the brain by penetrating the skin and underlying tissues. Electroencephalography (EEG) was used as a method to study how the fish responded to different stunning methods. That is, the baseline EEG of the fish was collected before any stunning was applied, and the fish then underwent one of four different stunning protocols. These were i) mechanical stunning by shooting the fish on the head with a non-penetrating bolt gun driven by pressurized air, ii) electrical stunning where both short electrical stunning (1 s) and longer electrical stunning (30 s) were tested, iii) live chilling where the fish was rapidly cooled down by adding ice cold water to tank, replacing the warm water immersing the fish iv) a combinational approach where electrical stunning for 30 s was followed by direct throat cutting and cooling. Before, during and after the stunning procedure, the fish were observed to note differences and/or deviations in behavior and physiological expressions (for example ventilation) and the EEG was measured.

• 2021-01-31 - 2021-04-30

• Numeric
• Text

• Oreochromis niloticus (linnaeus, 1758)

Laboratory experiment

The EEG was collected using electrodes that picked up the electrical activity in the brain. By stimulating the fish with a repetitive visual stimulus, responses to the visual stimulus were evoked if the fish was conscious, while these responses were absent if the fish was unconscious. The repetitive stimuli used here was a light that was turned on and off at a frequency of 2 Hz whose duration (i.e., the time the light was turned on) was 5 ms per event. In order to provide the right conditions for being stimulated by the lamp, the room was otherwise kept dark. Multiple measurements (between xx-yy measurements) were overlapped to generate the average response over a period of time. The response during this time was in turn divided into two parts, each 200 ms long: the 200 ms long light phase (which revolved around the 5 ms of light turned on) and the 200 ms long dark phase, which followed the 200 ms light phase (and thus occurred before the lamp was lit for 5 ms). Data were collected using a Powerlab and the signal was amplified by a BioAmp. The data was extracted from Labchart and transferred to excel for further analyses and calculations.

2021-01-31 - 2021-04-30

• Swedish University of Agricultural Sciences

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Departement of Applied Animal Science and Welfare

• Albin Gräns - Swedish University of Agricultural Sciences - Departement of Applied Animal Science and Welfare

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• Jeroen Brijs - Swedish University of Agricultural Sciences - Department of Applied Animal Science and Welfare

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• Institutionen för Biologi och miljövetenskap

• Institutionen för Biologi och miljövetenskap

Gothenburg - 5.8.18-12466/2018

• Zoology
• Behavioural sciences biology
• Fish and aquacultural science

• Agricultural and aquaculture facilities
• Electroencephalography

• consciousness
• Grand mal
• unconsciousness
• nile tilapia
• Live chilling
• Electrical stunning
• Stunning
• Percussive stunning
• Visually evoked responses
• Brain activity