Data for: "Host dependent specialized metabolism of nitrogen export in actinorhizal nodules induced by Frankia cluster-2"
https://doi.org/10.5878/wxkv-s592
The datafiles contain protein model structures as .pdb file format (Protein Data Bank) for the enzyme "2-oxoglutarate dehydrogenase/decarboxylase" of different Frankia species, in relation to the publication. These protein models were generated in silico on SWISS-Model website, using the crystal structure of Mycobacterium smegmatis (reference A0R2B1) and Staphylococcus epidermis (reference Q5HPC6) are templates. The modeling was performed using standard parameters, as described by the website instructions. The input data, namely amino acid sequences, were pulled from The National Center for Biotechnology Information (NCBI, Genbank) using a pBLAST search to identify the amino acid sequence from different Frankia species. The corresponding references to find these sequences are available as documentation files and in the supplementary data of the associated manuscript on the journal website. Each of the .pdb files contains information of one Frankia species, and is named after the corresponding species. For example, the datafile Falni refers to the model built for the protein of Frankia alni. These .pdb files are made available for those wishing to verify our conclusions. We recommend using protein visuallisation software, such as ChimeraX to open and visualise the data. The visualisation of the models are given in the supplementary figures.
Data files
Data files
Documentation files
Documentation files
Citation and access
Citation and access
Data access level:
Creator/Principal investigator(s):
Research principal:
Principal's reference number:
- SLU.vpe.2024.4.4.IÄ-8
Data contains personal data:
No
Citation:
Language:
Copyright:
Data from the SWISS model is covered by CC BY SA.
Method and outcome
Method and outcome
Time period(s) investigated:
Data format/data structure:
Species and taxons:
Data collection - Web-based experiment
Data collection - Web-based experiment
Mode of collection:
Web-based experiment
Description of the mode of collection:
In silico protein modeling: we uploaded existing data, namely amino acid sequences, onto the SWISS-model portal. Here the software predicts the 3D structure of the protein. The data was then downloaded as .pdb files which can be used in special software to open and visualise the protein strucure.
Time period(s) for data collection:
2021-09 - 2022-03
Source of the data:
- Research data: Published
- Research data
Geographic coverage
Geographic coverage
Geographic description:
The model organisms are occuring everywhere across the globe, and specified geographic location is therefore irrelevant. The dataset, description, and analyses are not impacted by geographical location.
Administrative information
Administrative information
Responsible department/unit:
Department of Crop Production Ecology
Other research principals:
Funding
Funding
Funding agency:
- Swedish Research Council
Award number:
VR2012-03061
Funding agency:
- Swedish Research Council
Opens a new window at ror.org.
ROROpens in a new tab
Award number:
VR2019-05540
Topic and keywords
Topic and keywords
Standard för svensk indelning av forskningsämnen 2025:
Keywords:
Relations
Relations
Is source of:
Publications
Publications
Citation:
Berckx, F., Nguyen, TV., Hilker, R., Wibberg, D., Battenberg, K., Kalinowski, J., Berry, A., Pawlowski, K. (2024). Host dependent specialized metabolism of nitrogen export in actinorhizal nodules induced by Frankia cluster-2. Journal of Experimental Botany: erae446. https://doi.org/10.1093/jxb/erae446Opens in a new tab
