Data for: Prevalence and antimicrobial resistant Campylobacter spp. in broiler chicken carcasses and hygiene practises in informal urban markets in a low-income setting
https://doi.org/10.5878/p0p0-ag22
This project aimed to contribute to reduced spread of antimicrobial resistant (AMR) zoonotic bacteria in urban livestock production systems in low-income countries (LIC). This was done by providing new data on spread and diversity of antimicrobial resistant Campylobacter strains. Drivers to mitigate disease and reduce antimicrobial usage was also generated which will facilitate future initiatives for coordinated actions to reduce spread of AMR.
The project investigated the prevalence of Campylobacter on broiler chicken carcasses sold at informal markets around the capital Kampala in Uganda. Antimicrobial resistance of the isolated Campylobacter strains was analyzed against five different antibiotics: tetracycline, ciprofloxacin, streptomycin, erythromycin, and nalidixic acid. The data file contains 36 rows (Campylobacter strains) and 14 columns, and the data is explained in the documentation file "read_me_AMR_campylobacter."
The vendors selling the broiler chicken carcasses included in the study were interviewed orally using a questionnaire to gather information about their knowledge, practices, and routines related to food safety and food hygiene at the included markets. The data file contains 120 rows (respondents) and 41 columns (survey questions). The data is explained in the documentation file "read_me_food_safety_questionnaire_results."
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Citation and access
Citation and access
Access level:
Creator/Principal investigator(s):
- Pius Okello – Makerere University - Department of Biomolecular Resources and Biolab Sciences
Research principal:
Principal's reference number:
- SLU.hbio.2025.IÄ-1
Data contains personal data:
No
Citation:
Language:
Method and outcome
Method and outcome
Unit of analysis:
Population:
The population consists of broiler chickens sold at informal markets in Kampala, Uganda, as well as the vendors.
Time method:
Study design:
- Observational study
Description of study design:
This study conducted in Kampala, Wakiso, and Mukono districts, Uganda, involved collecting broiler chicken carcasses and interviewing market vendors. Ten markets per district were randomly selected from official lists. A total of 124 carcasses were sampled, sufficient to detect a 75% prevalence with 5% error and 80% confidence.
Four carcasses per market were analysed using ISO methods for Campylobacter occurrence. The isolated bacterial strains were confirmed using PCR and identified to species level. All bacterial strains were also tested for antimicrobial resistance using the Disc Diffusion test.
Information about the vendors' knowledge of foodborne diseases, hygiene practices, and attitudes toward food hygiene was collected orally using a questionnaire consisting of 34 questions.
Sampling procedure:
Description of sampling:
The sample size was determined using an expected prevalence of 75%, with an estimated error of 5% and a confidence level of 80%. The sample size was set to 120. Four chicken carcasses per market were obtained, one from each vendor. In total, 30 markets were included. Information about the vendors' knowledge of foodborne diseases, hygiene practices, and attitudes toward food hygiene was collected orally using a questionnaire consisting of 41 questions.
Time period(s) investigated:
Variables:
48
Number of individuals/objects:
120
Response rate/participation rate:
100%
Description of the response rate/participation rate:
All selected vendors participated in the study.
Data format/data structure:
Data collection - Face-to-face interview
Data collection - Face-to-face interview
Mode of collection:
Face-to-face interview
Description of the mode of collection:
A written questionnaire was used to capture information on the included vendors’ knowledge and practices related to foodborne illness and antimicrobial resistance (AMR), focusing on the spread and mitigation of foodborne bacteria at informal markets. Chicken carcasses were bought from the interviewed vendors.
Time period(s) for data collection:
2022-02 - 2022-09
Data collector:
- Makerere University
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ROR
Sample size:
120
Number of responses:
120
Source of the data:
- Biological samples
Sample
Sample
Name:
chicken carcasses
Geographic coverage
Geographic coverage
Geographic location:
Geographic description:
Urban and periurban Kampala
Highest geographic unit:
County (NUTS 3)
Administrative information
Administrative information
Responsible department/unit:
Department of Animal Biosciences
Other research principals:
Funding
Funding
Funding agency:
- Swedish Research Council
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ROR
Award number:
2020-03631_VR
Award title:
Antimicrobial resistance in zoonotic pathogens – a challenge for urban small holder livestock production system
Funding information:
In low income countries (LIC) poultry production is important for livelihood and for food and nutrition security. Campylobacteriosis is one of the major bacterial public health risks globally and poultry meat is the most important route of transmission. In addition, AMR in Campylobacter is a growing concern. The project was conducted among informal food markets in Kampala, Uganda. Data was generated on food handlers’ knowledge and practices related to food hygiene and food safety focusing on spread and mitigation of AMR Campylobacter. The project contextualizes challenges with urban livestock production, AMR and antibiotic usage, and pathogenic zoonotic bacteria. It focuses on knowledge gaps related to spread of zoonotic bacteria resistant to antimicrobials at certain points in the poultry value chain. Generated results will likely be valid in other East African countries, as few studies have been published on AMR and Campylobacter in this region.
