Treatment with (R)-α-methylhistamine or IL4 stimulates mucin production and decreases Helicobacter pylori density in the murine stomach
Citering och åtkomst
Citering och åtkomst
Tillgänglighetsnivå:
Skapare/primärforskare:
- Macarena P. Quintana-Hayashi - Göteborgs universitet
Forskningshuvudman:
Data innehåller personuppgifter:
Nej
Citering:
Språk:
Metod och utfall
Metod och utfall
Population:
Möss delades in i olika grupper: icke-infekterad vehikelbehandlad kontroll, H. pylori-vehikelbehandlad, H. pylori-infekterad RaMH-behandlad och H. pylori-infekterad Il4-behandlad.
Tidsdimension:
Studiedesign:
- Preklinisk studie
Beskrivning av studiedesign:
Denna studie syftade till att återställa mucinproduktionen i infektionsmodellen av manlig C57BL/6 mus H. pylori (SS1) och utvärdera dess effekter på H. pylori-densiteten. Möss infekterade med SS1 behandlades med (R)-a-metylhistamin (RaMH) eller interleukin-4 (IL4).
Urvalsmetod:
Beskrivning av urval:
Totalt 300 µl per mus framställdes genom att lösa 2,6 mg GalNAz i 50 µl DMSO och späddes i PBS. GalNAz-injektionerna gavs intraperitonealt 14 dagar efter infektion, och möss avlivades 2 timmar efter injektionen genom cervikal dislokation under anestesi med isofluran. Helblodsprover från varje mus samlades in vid avlivning. Därefter skördades magarna och öppnades längs den större krökningen följt av en försiktig tvätt i steril PBS för att avlägsna chyme. En remsa av magsäckens mindre krökning innehållande förmage, corpus och antrum fixerades i buffrad formaldehyd 4% vattenlösning i 24 timmar. Den fixerade vävnaden var paraffininbäddad och histologiska objektglas preparerades med 4 µm tjocka vävnadsband. Dessutom samlades två delar av korpusen (ungefär 4 x 4 mm) upp. En placerades i RNA-later och lagrades vid 4°C, den andra frystes omedelbart i torris. Båda proverna förvarades senare vid -80°C fram till användning.
Tidsperiod(er) som undersökts:
Variabler:
26
Antal individer/objekt:
86
Datainsamlingar - 4 insamlingar
Datainsamlingar - 4 insamlingar
Datainsamling - Mätningar och tester
Datainsamling - Mätningar och tester
Insamlingsmetod:
Mätningar och tester
Beskrivning av insamlingsmetod:
A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.
Tidsperiod(er) för datainsamling:
2021-04-22 - 2021-05-06
Datakälla:
- Biologiska prover
Datainsamling - Mätningar och tester
Datainsamling - Mätningar och tester
Insamlingsmetod:
Mätningar och tester
Beskrivning av insamlingsmetod:
A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.
Tidsperiod(er) för datainsamling:
2021-11-17 - 2021-12-02
Datakälla:
- Biologiska prover
Datainsamling
Datainsamling
Beskrivning av insamlingsmetod:
A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.
Tidsperiod(er) för datainsamling:
2022-03-24 - 2022-04-09
Datakälla:
- Biologiska prover
Datainsamling
Datainsamling
Beskrivning av insamlingsmetod:
A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.
Tidsperiod(er) för datainsamling:
2022-09-07 - 2022-09-22
Datakälla:
- Biologiska prover
Administrativ information
Administrativ information
Ansvarig institution/enhet:
Avdelningen för medicinsk kemi och cellbiologi
Etikprövning:
Etikprövningsmyndigheten - 52-2021
The Göteborgs Djurförsöksetiska Nämnd (Ethic No. 52-2021) approved all experimental procedures based on the regulation from Djurskyddsförordningen DFS 2004:4.
Ämnesområde och nyckelord
Ämnesområde och nyckelord
CESSDA Topic Classification:
Standard för svensk indelning av forskningsämnen 2025:
Publikationer
Publikationer
Citering:
Citering:
Citering:
Metadata
Metadata
