Raw scRNA-seq FASTQ-datafiler för: Nerve growth factor responsive elements modulate immune cell inflammation and are dysregulated in an Alzheimer's disease mouse model

• Data är öppet tillgängliga

• Maria Eriksdotter - Karolinska Institutet - Institutionen för neurobiologi, vårdvetenskap och samhälle

  Öppnar nytt fönster hos orcid.org.

  ORCID
• Ruchi Gera - Karolinska Institutet - Institutionen för neurobiologi, vårdvetenskap och samhälle

  Öppnar nytt fönster hos orcid.org.

  ORCID

• Karolinska Institutet

  Öppnar nytt fönster hos ror.org.

  ROR

• Creative Commons Public domain-dedikation (CC0 1.0)

• Engelska

• Djur

Vild typ Mus musculus C57BL/6J laboratoriemöss; single-cell-suspensioner från splenocyter använda för scRNA-seq-analys.

• Experimentell studie
• Preklinisk studie

Preklinisk experimentell studie med vildtyp C57BL/6J-möss. Splenocyter isolerades och processades för single-cell RNA-sekvensering (scRNA-seq) för att generera transkriptomiska profileringsdata.

2 biologiska prover

• Övrigt

Laboratorieexperiment

Mice were kept on a 12h light/dark cycle and food and water were available ad libitum. Mice were deeply anesthetized using isoflurane to collect spleen. Dissected spleens were processed to collect splenic immune cells. After isolation, the splenocytes were counted and the viability was measured with the Luna FL dual fluorescence cell counter with dual stain Acridine Orange/Propidium Iodide, with cell size gated between 1-60 μm (Logos Biosystems). Cell suspensions with viability of 85% were encapsulated using the Next Gem Single Cell 3′ Reagent kit v3.1. (Dual index) from 10x Genomics (cat: PN-100026), aiming to capture 5,000 cells. Libraries were prepared as per manufacturer’s instruction (protocol CG000315) and sequenced using the Illumina platform on a Next seq2000 P3 100 cycles flow cell, aiming for an average read depth of 40,000 reads/cell. The cell capture and library generation were performed at the Single Cell Core Facility of Flemingsberg Campus (SICOF) that receives funding from the Infrastructure Board at Karolinska Institutet.

Institutionen för neurobiologi, vårdvetenskap och samhälle

• Specifika sjukdomar, störningar och medicinska tillstånd

• Immunologi

• Inflammation
• Nervtillväxtfaktorer
• Kolinerga neuroner
• Fenotypbestämning av immuncell