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RNA-Seq data from Ovarian Tumours

https://doi.org/10.17044/SCILIFELAB.28342484

The data presented in this item contain sensitive information that cannot be shared openly. Work on depositing the data in FEGA Sweden has been initiated. FEGA Sweden is a national node of the Federated European Genome-phenome Archive (FEGA), which allows data to be shared under controlled access. The datasets in FEGA Sweden are findable through the European Genome-phenome Archive web portal (https://ega-archive.orgÖppnas i en ny tabb). This dataset refers to the raw data used in the preprint "Deep plasma proteomics identifies and validates an eight-protein biomarker panel that separate benign from malignant tumors in ovarian cancer" available on MedRxiv (DOI: 10.1101/2024.10.10.24315232v1) Data Set Description Samples: 81 fresh frozen tumor tissue samples, either benign or malignant, from women with suspected ovarian cancer were used for analysis of mRNA expression. Library Preparation and Sequencing: Sequencing libraries were prepared from 122-194 ng (three samples), 200 ng (seven samples) or 500 ng (71 samples) total RNA using the TruSeq stranded mRNA library preparation kit (cat# 20020595, Illumina Inc.) including polyA-selection. The libraries were then sequenced on a NovaSeq 6000 system (Illumina Inc.) on S4 flowcells with version 1.5 sequencing chemistry on three lanes. Paired-end sequencing of with read lengths of 150 bp was used. Library preparation and sequencing was performed by the SNP&SEQ Technology platform, SciLifeLab, National Genomics Infrastructure Uppsala, Sweden.

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https://doi.org/10.17044/SCILIFELAB.28342484

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