Svampar i Sverige

A collection of forest soil samples collected sampling was carried out in connection with the Swedish Forest Soil Inventory during 2014–2016. Organic topsoil samples were collected in 3 m2 plots in 359 conifer-dominated forest stands distributed across the Swedish boreal biome from 60° to 68° N and from 12° to 24° E. ITS2 markers were amplified from extracted DNA using the fungal-specific primer gITS7 and a 3:1 mix of the reverse primers ITS4 and ITS4arch, with both forward and reverse primers fitted with unique 8 bp sample identification tags. Composite amplicon samples were sequenced on the RSII platform (Pacific Biosciences) by SciLifeLab NGI (Uppsala, Sweden) after the addition of sequencing adaptors by ligation. This dataset was published via the SBDI ASV portal (https://asv-portal.biodiversitydata.se)