MINFLUX data for Na,K-ATPase membrane clustering analysis
https://doi.org/10.71775/kth.vsztr-nq320
Data belonging to the preprint https://doi.org/10.64898/2026.06.30.735534
Raw MINFLUX data for a study of Na,K-ATPase clustering in the plasma membrane of epithelial cells. Two subunits, alpha1 and beta1 were tagged with EGFP and imaged using DNA-PAINT.
The msr files contain the localizations and metadata and can be opened using imspector software.
Imspector is a commercial software developed by Abberior Instruments used for operating the Abberior MINFLUX microscope, specifically running acquisitions and exporting the data. Find out more about the software: https://abberior.rocks/superresolution-confocal-systems/imspector . Software is installed and run on Windows OS and the versions used to generate the data are vol. m2205 and m2410
Imspector software is not freely available, however, .msr files can be exported as .mat or .npy and further processed using either custom matlab or python scripts.
Abbreviations:
MINFLUX - minimal fluorescence photon fluxes microscopy
EGFP - enhanced green fluorescent protein
DNA-PAINT - DNA Points Accumulation for Imaging in Nanoscale Topography
Naming convention:
e.g. 1_NKA_A1_EGFP
1 - Image number
NKA - Na,K-ATPase (the studied protein)
A1_EGFP - the protein subunit tagged with EGFP (either alpha 1 or beta 1)
Gå till källa för data
https://doi.org/10.71775/kth.vsztr-nq320
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oai:
oai:datarepository.kth.se:vsztr-nq320
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Finansiär:
- Swedish Research Council
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2023-00163
Projektnamn på ansökan:
National Microscopy Infrastructure, NMI
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Standard för svensk indelning av forskningsämnen 2025:
Nyckelord:
- minflux
- microscopy
- Na,K-ATPase
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