TARGET-seq genotyped single-cell RNA sequencing of hematopoietic stem cells and megakaryocyte-erythroid progenitor cells from a dual SF3B1-mutant MDS-RS patient and 3 healthy donors

Eva Hellström-Lindberg

doi:10.48723/d7s9-6336

TARGET-seq genotyped single-cell RNA sequencing of hematopoietic stem cells and megakaryocyte-erythroid progenitor cells from a dual SF3B1-mutant MDS-RS patient and 3 healthy donors

https://doi.org/10.48723/d7s9-6336

This dataset consists of TARGETseq (genotype-targeted plate-based SmartSeq2) single-cell RNA sequencing data of purified hematopoietic stem cells (HSC) and megakaryocyte-erythroid progenitors (MEP) from the bone marrow of a dual SF3B1-mutant MDS-RS patient (Patient 1 in the associated publication) over 2 timepoints (39 months post-diagnosis, with dominant SF3B1-N626D, and 118 months post-diagnosis, with dominant SF3B1-K666N); and purified HSC/MEP from the bone marrow of three healthy donors. The objective of this data collection was to assess the molecular characteristics that increase fitness in SF3B1-mutant HSC as compared to normal HSC. The dataset is approximately 187 GB and includes the file types: zip, xlsx, bam, bam.bai, rds.

Download 2 documentation files (3.49 KiB)

Documentation files

Citation and access

Method and outcome

Administrative information

Topic and keywords

Publications

Metadata

Version 1

Karolinska Institutet

TARGET-seq genotyped single-cell RNA sequencing of hematopoietic stem cells and megakaryocyte-erythroid progenitor cells from a dual SF3B1-mutant MDS-RS patient and 3 healthy donors

Documentation files

Citation and access

Creator/Principal investigator(s):

Research principal:

Data contains personal data:

Type of personal data:

Code key exists:

Sensitive personal data:

Citation:

Data access level:

License:

Language:

Method and outcome

Unit of analysis:

Population:

Time method:

Study design:

Sampling procedure:

Description of sampling:

Data format/data structure:

Samples/material - Existing from scientific collection/biobank

Name:

Type(s) of sample:

Administrative information

Responsible department/unit:

Contributor(s):

Ethics Review:

Funding

Funding agency:

Award number:

Funding agency:

Award number:

Funding agency:

Award number:

Funding agency:

Award number:

Topic and keywords

Standard för svensk indelning av forskningsämnen 2025:

Keywords:

Publications

Citation:

DOI:

Metadata