Skip to main content
Researchdata.se

DNA sequencing data for "Stable clonal contribution of lineage-restricted stem cells to human hematopoiesis"

https://doi.org/10.48723/313d-dd68
This dataset contains three types of DNA sequencing data. -Error-corrected DNA capture sequencing (ECTS) -Bulk whole-exome sequencing (WES) -Single-colony whole-genome sequencing (WGS) All sequencing was performed on an Illumina NovaSeq 6000 at the National Genomics Infrastructure in Stockholm, using paired-end sequencing mode. ECTS Bone marrow mononuclear cells isolated from all 93 healthy donors were subjected to ECTS for identification of somatic mutations targeted to 23 genes encompassing the most recurrently mutated genes reported in clonal hematopoiesis. WES BM MNC DNA isolated from the first visit from 20 healthy donors above 71 years was subjected bulk WES. Paired buccal swab DNA was used for normal controls. Single colony WGS DNA extracted from 333 genotyped single colonies and 10 control buccal swabs from 10 donors was subjected to WGS. The dataset consists of three folders: scWGS contains 343 files in CRAM format, totaling approximately 16.3 TiB (17.9 TB). WES contains 40 files in CRAM format, totaling approximately 650 GiB (710 GB). ECTS contains 117 files in CRAM format, totaling approximately 18 GiB (20 GB).
Download documentation (5 files / 217.9 KiB)

Documentation files

  • CRAM_file_description.xlsx
    27.43 KiB
  • ECTS_cram_to_upload_md5_file_size.txt
    7.11 KiB
  • README.pdf
    156.83 KiB
  • scWGS_cram_to_upload_md5_file_size.txt
    23.67 KiB
  • WES_cram_to_upload_md5_file_size.txt
    2.86 KiB

Citation and access

Data access level:

Creator/​Principal investigator(s):

Research principal:

Data contains personal data:

Yes

Type of personal data:

Genetic data (Germline single nucleotide polymorphisms and somatic mutations)

Code key exists:

Yes

Sensitive personal data:

Yes

Citation:

Language:

Method and outcome

Population:

Healthy individuals with rigorous inclusion criteria ensuring the absence of current or previous blood disorders or cancer and without ongoing treatment with immunosuppressive or other drugs likely to affect hematopoiesis.

Time method:

Study design:

  • Experimental study

Sampling procedure:

Time period(s) investigated:

Number of individuals/​objects:

93

Data format/​data structure:

Samples/material Collected from scientific collection/biobank

Name:

Karolinska Institutet MDS Biobank

Geographic coverage

Geographic location:

Administrative information

Responsible department/​unit:

Department of Medicine, Huddinge [H7]

Ethics Review:

Stockholm - 2018/901-31

Studies of hereditary and acquired disease mechanisms in myelodysplastic and myelodysplastic / myeloproliferative syndrome, MDS-related AML and adjacent hematological conditions with a need for improved differential diagnosis. The last updated version is 2025-01131-02 (Stockholm).

Funding

Funding agency:

Award number:

KAW 2016.0105, 2015.0195

Funding agency:

  • Tobias Foundation

Award number:

4-1122/2014

Funding agency:

Funding agency:

Award number:

613/06

Funding agency:

Award number:

538-2013-8995, 2015-03561

Funding agency:

Award number:

MC_UU_12009/5

Funding agency:

Award number:

22 2178 Pj

Funding agency:

Award number:

224132, 244282

Funding agency:

  • Dr Åke Olsson Foundation

Award number:

2021-00087

Topic and keywords

Standard för svensk indelning av forskningsämnen 2025:

Keywords:

Publications

Citation:

Yoshizato, T., Nilsson, C., Grasso, F., Högstrand, K., Mazzi, S., Winroth, A., Lehander, M., Barbosa, I., Waldin, G., Mortera-Blanco, T., Yoshizato, T., Nilsson, C., Grasso, F., Högstrand, K., Mazzi, S., Winroth, A., Lehander, M., Barbosa, I., Waldin, G., & Mortera-Blanco, T. (2025). Stable clonal contribution of lineage-restricted stem cells to human hematopoiesis. In NATURE GENETICS. https://doi.org/10.1038/s41588-025-02405-wOpens in a new tab

SwePub:

oai:swepub.ki.se:1076513Opens in a new tab

Contact

Metadata

Version 1
doris
ki
Published:|Updated: